Re: [Histonet] Clostridium difficile - what do you do

2011-12-03 Thread Rob Geske
Hi Brett,   you might want to take a look at the publication linked to below.  2% glutaraldehyde is indicated in some of the (many) references from the publication and you might consider that option (spiking your NBF with 2% glut) if it will not interfere with any of the procedures you have

Re: [Histonet] Re: Clostridium difficile - what do you do

2011-12-03 Thread Bob Richmond
It's "difficile" rather than *difficilis because it's a third declension singular neuter adjective, in apposition with "Clostridium", a second declension neuter. A reference to the Oxford-BBC Guide to Pronunciation suggests that my pronunciation is the correct one in English. Every European langua

Re: [Histonet] Re: Clostridium difficile - what do you do

2011-12-03 Thread John Kiernan
The school Latin pronunciation (classical rather than ecclesiastical) would be dif-ik-il-ay, but changing the k to s would be a reasonable anglicization, as is often done to make Latin names of plants and animals euphonious to anglophones. The name of this bacterium seems to be grammatically wro

[Histonet] As to "difficile" pronunciation.

2011-12-03 Thread Rene J Buesa
"Difficile" is italian for "difficult" and it is pronounced: difi-CHI-le" the "CHI" as in "cheese". René J. --- On Sat, 12/3/11, Bob Richmond wrote: From: Bob Richmond Subject: [Histonet] Re: Clostridium difficile - what do you do To: histonet@lists.utsouthwestern.edu Date: Saturday, December

Re: [Histonet] cassette marker

2011-12-03 Thread joelle weaver
I like the securline marker II/superfrost. Used for years stay through reagents, AR, decal Sent from my Verizon Wireless BlackBerry -Original Message- From: histot...@imagesbyhopper.com Date: Sat, 3 Dec 2011 18:59:45 To: Cc: Subject: Re: [Histonet] cassette marker I like the StatLab m

Re: [Histonet] cassette marker

2011-12-03 Thread histot...@imagesbyhopper.com
I like the StatLab markers. On Dec 3, 2011, at 1:38 PM, "Rathborne, Toni" wrote: Can anyone recommend a marker for using on cassettes? We currently use pencil, which sometimes smudges. We've tried a few markers already, but some fade, while others hold up well for processing, but won't when

[Histonet] cassette marker

2011-12-03 Thread Rathborne, Toni
Can anyone recommend a marker for using on cassettes? We currently use pencil, which sometimes smudges. We've tried a few markers already, but some fade, while others hold up well for processing, but won't when placed in decalcifier. Vendors are welcome to respond. Thanks, Toni CONFIDENTIALIT

[Histonet] Re: Clostridium difficile - what do you do

2011-12-03 Thread Bob Richmond
I have no idea - possibly no special precautions - I'd address this question - about Clostridium difficile - to the right person at the CDC. They'll know. While on the subject, how do you pronounce "difficile"? I say dih-FISS-illy - mostly on the basis of how Mozart pronounced it in his comic mote

Re: [Histonet] For H&E staining - TBS SHUR/Stain or Leica Autostainer XL

2011-12-03 Thread Rene J Buesa
You can dewax and hydrate your immunos much easier: use a 2% aq. vol:vol solution of dishwasher soap followed by tap and distilled water. As simple as that! The method has been tested and validated for immunos and is standard procedure of some labs. If you want I can provide you with copies of