[Histonet] Invitation to connect on LinkedIn

2012-01-30 Thread Judith McKinney via LinkedIn
LinkedIn Judith McKinney requested to add you as a connection on LinkedIn: -- David, I'd like to add you to my professional network on LinkedIn. - Judith Accept invitation from Judith McKinney http://www.linkedin.com/e/yvpgd1-gy1l

[Histonet] Need feed back from any users out there!

2012-01-30 Thread Colleen Forster
Hello Histoland, Can anyone who may have used or is using this piece of equipment give me their feed back? We are considering it to do an "online" dewaxing and retrievel with the Biocare Nemesis stainer. Thanks in advacne for your comments! Colleen Forster HT(ASCP)QIHC U of MN Lab

Re: [Histonet] Need feed back from any users out there!

2012-01-30 Thread Kim Merriam
I wouldn't put xylene on my Nemesis stainer, it might ruin some of the parts of the instrument.  Also, the Nemesis is all room temp, you can't do HIER on it (but you can do enzymes on it).   My 2-cents. Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Coll

[Histonet] Carbon 14

2012-01-30 Thread jstaruk
Does anyone have any experience with tissue injected with carbon 14? I have some questions about precautions and disposal. Thanks Jim ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com ___ Histonet mailing

[Histonet] Processing adipose tissue

2012-01-30 Thread David Burk
Esteemed experts, We have many clients who want to process mouse and human adipose tissue and are having some quality issues in the resultant slides. We have tried processing small chunks of tissue (<1 cm x 0.5 cm) on our automated processor (Excelsior) as follows: Tissue fixed for ~24 hrs

Re: [Histonet] Carbon 14

2012-01-30 Thread Rene J Buesa
Usually the amounts are very small, but always treat the tissues with the adequate precautions. Your radiology department can inform you of the current regulations in your area. René J. --- On Mon, 1/30/12, jstaruk wrote: From: jstaruk Subject: [Histonet] Carbon 14 To: "'Histonet'" Date: Mo

[Histonet] Interviewing Histotechs...

2012-01-30 Thread Jerry Ricks
I gather it is different in clinical labs than in research labs. In clinical labs there is an emphasis on quantity and speed. In research the emphasis is on doing good experiments. Our "patients" are almost always deceased or shortly about to be so there is no urgency of diagnosis factor. F

[Histonet] SSTR1 - 5 antibody

2012-01-30 Thread Gudrun Lang
Hi! Can someone give a recommendation for SSTR1 to SSTR5 antibodies for human FFPE immunohistochemistry? synonym: Somatostatin-Receptor 1 many thanks Gudrun Lang histolab, Linz, Austria ___ Histonet mailing list Histonet@lists.utsouthwestern.e

[Histonet] Great Louisiana Positon

2012-01-30 Thread Hale, Meredith
Great opportunity for a Histotechnician in a brand new laboratory! Acadiana Gastroenterology Associates, LLC, a six (6) Physician Practice located in Lafayette, Louisiana, is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certi

Re: [Histonet] Interviewing Histotechs...

2012-01-30 Thread koellingr
I hope there is at least a bit more to clinical histology than quantity and speed. Maybe I know the particular high schooler you mentioned who was so good since I mentored that Mercer program/class for years and years and even brought in class-wide IHC hands-on projects. Before the teacher left

[Histonet] Immunofluorescence staining/minimizing background staining

2012-01-30 Thread Kasai, Miki (NIH/NCI) [E]
Hi, We are performing some immunofluorescence staining on mouse lung tissue. We are getting some nice positive staining with some of our initial antibodies (procollagen, cytokeratin). We would like to minimize the amount of background staining we are getting. We are titering our primary antibodi

[Histonet] Thank you

2012-01-30 Thread Tim Wheelock
Hi All: I want to thank everyone for the advice on xylene-resistant gloves. It was really helpful. Tim Wheelock Tim Wheelock Assistant Director Harvard Brain Tissue Resource Center Instructor In Neuroanatomy 203 Mailman Research Center McLean Hospital Belmont MA 02478 Phone: 617-855-3592 Fax:

RE: [Histonet] Processing adipose tissue

2012-01-30 Thread Jerry Ricks
Hi David, 21 hours in isopropyl seems likw ea lot, and I don't see any "clearing step to remove the IPA." I've been using Slide Brite instead of Xylene, but I see that Rene Buesa published a study indicating that mineral oil is the cat's meow for xylene substitutes. http://www.google.com/url

Re: [Histonet] interview cutting-OT-disarmingly long for deletiondisinterested

2012-01-30 Thread Kim Donadio
Oh come on. The truth of the matter of why I like to give a manual test to new hires is because people are graduating some Internet programs without the technical skills to function in a lab. Not all. But I've seen a lot. Just saying:) I don't think it should be made a big deal. You take a dr