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David,
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Accept invitation from Judith McKinney
http://www.linkedin.com/e/yvpgd1-gy1l
Hello Histoland,
Can anyone who may have used or is using this piece of equipment give me
their feed back? We are considering it to do an "online" dewaxing and
retrievel with the Biocare Nemesis stainer. Thanks in advacne for your
comments!
Colleen Forster HT(ASCP)QIHC
U of MN
Lab
I wouldn't put xylene on my Nemesis stainer, it might ruin some of the parts of
the instrument. Also, the Nemesis is all room temp, you can't do HIER on it
(but you can do enzymes on it).
My 2-cents.
Kim
Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA
From: Coll
Does anyone have any experience with tissue injected with carbon 14? I have
some questions about precautions and disposal.
Thanks
Jim
___
James E. Staruk HT(ASCP)
www.masshistology.com
www.nehorselabs.com
___
Histonet mailing
Esteemed experts,
We have many clients who want to process mouse and human adipose tissue
and are having some quality issues in the resultant slides.
We have tried processing small chunks of tissue (<1 cm x 0.5 cm) on our
automated processor (Excelsior) as follows:
Tissue fixed for ~24 hrs
Usually the amounts are very small, but always treat the tissues with the
adequate precautions. Your radiology department can inform you of the current
regulations in your area.
René J.
--- On Mon, 1/30/12, jstaruk wrote:
From: jstaruk
Subject: [Histonet] Carbon 14
To: "'Histonet'"
Date: Mo
I gather it is different in clinical labs than in research labs. In clinical
labs there is an emphasis on quantity and speed. In research the emphasis is
on doing good experiments. Our "patients" are almost always deceased or
shortly about to be so there is no urgency of diagnosis factor. F
Hi!
Can someone give a recommendation for SSTR1 to SSTR5 antibodies for human
FFPE immunohistochemistry?
synonym: Somatostatin-Receptor 1
many thanks
Gudrun Lang
histolab, Linz, Austria
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Histonet mailing list
Histonet@lists.utsouthwestern.e
Great opportunity for a Histotechnician in a brand new laboratory!
Acadiana Gastroenterology Associates, LLC, a six (6) Physician Practice
located in Lafayette, Louisiana, is looking for a certified HT or HTL to
run their newly constructed laboratory. Candidate must be ASCP certified
and CLIA certi
I hope there is at least a bit more to clinical histology than quantity and
speed. Maybe I know the particular high schooler you mentioned who was so good
since I mentored that Mercer program/class for years and years and even brought
in class-wide IHC hands-on projects. Before the teacher left
Hi,
We are performing some immunofluorescence staining on mouse lung tissue. We
are getting some nice positive staining with some of our initial antibodies
(procollagen, cytokeratin).
We would like to minimize the amount of background staining we are getting.
We are titering our primary antibodi
Hi All:
I want to thank everyone for the advice on xylene-resistant gloves.
It was really helpful.
Tim Wheelock
Tim Wheelock
Assistant Director
Harvard Brain Tissue Resource Center
Instructor In Neuroanatomy
203 Mailman Research Center
McLean Hospital
Belmont MA 02478
Phone: 617-855-3592
Fax:
Hi David,
21 hours in isopropyl seems likw ea lot, and I don't see any "clearing step to
remove the IPA."
I've been using Slide Brite instead of Xylene, but I see that Rene Buesa
published a study indicating that mineral oil is the cat's meow for xylene
substitutes.
http://www.google.com/url
Oh come on. The truth of the matter of why I like to give a manual test to new
hires is because people are graduating some Internet programs without the
technical skills to function in a lab. Not all. But I've seen a lot. Just
saying:)
I don't think it should be made a big deal. You take a dr
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