Hello,
Happy Friday.
We are currently running IHC on Ventana XT and Ultra instrumentation.
We recently in the past couple of months have seen a rise in tissue
section loss. Can anyone share with me their protocol for drying and
baking slides prior to IHC automated staining??
We had this problem
I just wanted to thank everyone who responded to my VIP 6 inquiry. I really
appreciated the assistance and we are setting up a demo.
Have a great weekend everyone !!
Vicki Gauch
AMCH
Albany, NY
___
Histonet mailing list
What kind of slides are you using? I have had bad lot numbers of slides. New
lot number cleared up the problem.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From:
Really, the more important question is, if the lab is unwilling to provide
PPE in this case, where else are they lacking in looking after your safety?
I've never heard of using xylene without gloves (nitrile or latex). The
EHS department here at Pitt (academic research) would explode with horror
Good Morning,
During the summer and warm months, we ship blocks with a cold pack in the box.
Seems to work fine!!!
Wanda
WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC 29406
843-847-4586
843-847-4296 fax
This email and
LinkedIn
Pamela Marcum requested to add you as a connection on LinkedIn:
--
David,
I'd like to add you to my professional network on LinkedIn.
- Pamela
Accept invitation from Pamela Marcum
Sorry did mean to this on HistoNet!! Pam
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum
via LinkedIn
Sent: Friday, February 24, 2012 8:35 AM
To: David E.
Subject: [Histonet] Invitation
I used to send the overnight in one of those Styrofoam containers along with
those freeze and reuse plastic pouches inside.
René J.
--- On Thu, 2/23/12, E V hairlesstur...@gmail.com wrote:
From: E V hairlesstur...@gmail.com
Subject: [Histonet] Paraffin Block Shipping Question
To:
Over the counter is OK, even for bone marrow smears.
René J.
--- On Thu, 2/23/12, Fran Pearsall fpea...@clemson.edu wrote:
From: Fran Pearsall fpea...@clemson.edu
Subject: [Histonet] cytology slides
To: histonet@lists.utsouthwestern.edu
Date: Thursday, February 23, 2012, 5:19 PM
Hi every one,
There is an extensive literature linking xylene with some types of cancers,
especially blood cancers, as well as with birth defects caused by it and many
other aromatic compounds.
The real solution is to just eliminate it, and that be done from ALL, and I
mean ALL, the histology phases where it
I was sent this link a couple of weeks ago, thought I would share it.
http://www.darkdaily.com/health-of-pathology-laboratory-technicians-at-risk-from-common-solvents-like-xylene-and-toluene-070511#axzz1hkVgUzSf
Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer.
Your drying protocol seems to be adequate. Perhaps your (+) slides are old and
in that case they tend to lose their adhesion.
René J.
--- On Fri, 2/24/12, Kuhnla, Melissa melissa.kuh...@chsli.org wrote:
From: Kuhnla, Melissa melissa.kuh...@chsli.org
Subject: [Histonet] Tissue adhesion
To:
Greetings!
I am looking for staining methods applicable to thick sections of nervous
tissue for confocal microscopy.
We would like to stain cell processes and use specialized software for
reconstruction of dendrites.
We do not have the capability to impale cells and impregnate them with for
Dear All,
I am using clear packing tape on mma embedded rabbit femurs with implants.
The reasoning for the use of tape is that the implant crumbles when being
cut without the tape. I have switched to a new tape that partially comes
off with xylene, but does not release from the section on the
Please reply to all. I am interested in this info. also.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of M.O.
Sent: Friday, February 24, 2012 1:30 PM
To: histonet@lists.utsouthwestern.edu
Subject:
Does anyone have a Halon or Ansul Clean guard fire extinguisher in the lab?
The company that does our sprinkler system has suggested that we have that kind.
Thanks,
Linda
Our Vision: To be the #1 choice for all your GI services
Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Linda
We do, we have it located where our server is, its only supposed to be used on
our server, we have regular A B C extinguishers in the lab. The Halon ones are
for computers and stuff like that. They are not inexpensive, they cost about
$185.00 each. They are liquid that does not damage
Thanks Liz,
The guy that was in here today said we should use the Halon or Ansul Cleanguard
extinguishers on any piece of equipment to reduce the damage from the ABC
extinguishers. I know we have Halon in the server room but I've never heard of
having one in the lab itself. He was also
Use of halon extinguishers Do not used in a small or closed space.
The way it works is by rapidly depleting oxygen available for the fire.
May well do the same for you.
Just sayin.. Machinery is replacable.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Be careful with Halon. We at Ford used in in our server rooms, but we had
many hundreds of thousands of dollars of servers and a fairly large room.
If it is set off, EVERYBODY MUST leave the room. Halon will NOT support
life
(this is why it works as a fire suppressor). So, along with the
We are looking for help with our grossing. Can you help? You must be CLIA
qualified to do the high complexity testing we know as grossing. Times have
changed as we used to do this work ourselves, but no more. Two of my staff
qualify but I need another.
We are a small laboratory in Marin County,
Preserve the profession - hire an ASCP Certified PA.
Sent from my Windows Phone
From: Bruce Gapinski
Sent: 2/24/2012 1:52 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Grossing techs
We are looking for help with our grossing. Can you help? You must be
CLIA qualified to do the high
Hi,
Please we are having problems getting great slides from tissue/biopsy
specimens like gastric, esophageal, needdle biopsy and Liver Biopsy. Our
pathologists are not happy at all because of this situation.
I will really, really appreciate if you guys in histoland could suggest
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