Hi Luis,
I had good success with mouse monoclonal clone A-Gel2 VC2 from
Neomarkers after HIER in citrate buffer. Please, check my paper: Lhotak
S. et al, Clin Exp Metastasi 2000, 18(6): 463-70, for conditions, and
for staining for other MMPs and TIMPS. It is a bit dated, though, there
may be other
We use DI water in our waterbaths and have hardly problems with the adhesion
of tissue on charged slides. - this may also be an advantage, because the
counter-ions don't bind to each other. And the positive charge slide-surface
isn't saturated.
Gudrun
-Ursprüngliche Nachricht-
Von: hist
Yes. Tap water may contain suspended particle that can be transferred to the
slide.
René J.
--- On Fri, 3/16/12, Amber McKenzie wrote:
From: Amber McKenzie
Subject: [Histonet] water bath
To: "histonet@lists.utsouthwestern.edu"
Date: Friday, March 16, 2012, 5:58 PM
Is there a difference in