[Histonet] MMP2

2012-03-17 Thread Sarka Lhotak
Hi Luis, I had good success with mouse monoclonal clone A-Gel2 VC2 from Neomarkers after HIER in citrate buffer. Please, check my paper: Lhotak S. et al, Clin Exp Metastasi 2000, 18(6): 463-70, for conditions, and for staining for other MMPs and TIMPS. It is a bit dated, though, there may be other

AW: [Histonet] water bath

2012-03-17 Thread Gudrun Lang
We use DI water in our waterbaths and have hardly problems with the adhesion of tissue on charged slides. - this may also be an advantage, because the counter-ions don't bind to each other. And the positive charge slide-surface isn't saturated. Gudrun -Ursprüngliche Nachricht- Von: hist

Re: [Histonet] water bath

2012-03-17 Thread Rene J Buesa
Yes. Tap water may contain suspended particle that can be transferred to the slide. René J. --- On Fri, 3/16/12, Amber McKenzie wrote: From: Amber McKenzie Subject: [Histonet] water bath To: "histonet@lists.utsouthwestern.edu" Date: Friday, March 16, 2012, 5:58 PM Is there a difference in