[Histonet] re: Pin 4

2012-04-20 Thread Robert Jann
Karen, Here's our protocol for FFPE sections on the Benchmark XT. Be advised that the times will be approximate for you and that we use Dako P504s @ 1:50. Also, you can call Ventana and request a rep. to come to your site and help you optimize the stain. 1. Create/manage protocols 2.

[Histonet] Re: Pinning Specimen

2012-04-20 Thread Margaret Horne
Just another idea : I have used dental wax. Comes in a package of about 20 sheets pf pink wax, each 7.5 cm x 14.5 cm x 0.2 cm. It's a little bit more pliable than the waste paraffin, but not as cheap :-) I cut it in a long rectangle so that it sticks out of the formalin with the tissue at the

[Histonet] Part Time Job Opening, Springfield, MA

2012-04-20 Thread Amanda L
Please Send Resume for: Part Time opportunity for a Histotechnican. Duties and responsibilities include: • Under general supervision performs routine and non-routine activities involved in the preparation of slides, for microscopic evaluation by pathologist(s) according to policies and

[Histonet] Re: Histonet Digest, Vol 101, Issue 27

2012-04-20 Thread Eric Tambutte
Bonjour, Je suis absent du laboratoire jusqu'au jeudi 03 mai 2012. Je vous répondrai le plus rapidement possible. Eric Tambutté Thank you for your mail. I will be out of office till May 03rd 2012. I will respond to your e-mail as soon as possible. Thank you for your understanding. Best

[Histonet] Re: Pinning Specimens

2012-04-20 Thread Bob Richmond
About pinning specimens so they fix flat: Margaret Horne notes the use of dental wax. I've used it to make small boats for the rather exacting procedure of pinning muscle biopsy specimens for electron microscopy. Here a 1 to 2 mm bundle of longitudinal fibers has to be gently stretched to its

[Histonet] Immunofluorescence

2012-04-20 Thread Thotakura, Anil Kumar
Dear All, Can some one please send the protocol for immunofluorescence, I want to stain for FITC CD45.1 on mice spleens. Thank you very much for your help. Many Thanks, Anil kumar. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: [Histonet] GMS on Toenail

2012-04-20 Thread Britton, Josette C
The way we got around that problem was to do a manual PAS stain instead! Josie Britton HT Cheshire Medical Center Keene NH -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Thursday,

[Histonet] Leica Peloris Processor

2012-04-20 Thread Joe W. Walker, Jr.
Hello all, I am new to your listserve. I am wondering if anyone has experience with the Leica Peloris processor. Specifically, has anyone noticed if the higher processing temperatures has affected any IHC staining results or FISH results. Looking forward to responses, Joe W. Walker, Jr.

Re: [Histonet] Leica Peloris Processor

2012-04-20 Thread Richard Cartun
In my experience, no. Our current IHC/ISH is as good, if not better than what we were getting with our former processors. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital

Re: [Histonet] Leica Peloris Processor

2012-04-20 Thread Patrick Laurie
We have 4 peloris processors, run many IHC and FISH stains, none of them have had any negative effects due to the increased temperatures. The increased temps aren't that high, 45 degrees for all the liquids (we use xylene) and 65 degrees for the paraffins. Good luck Patrick On Fri, Apr 20,

[Histonet] Re: Histonet Digest, Vol 101, Issue 27

2012-04-20 Thread Amos Brooks
Hi, Strange issue with your Ki-67. I don't know what would do that unless it was non-specific background. Is it nuclear or cutoplasmic? If you are working in mice or other animals you could try labeling them with BRDu then detecting it with an anti BRDu antibody. Humans don't like being