Karen,
Here's our protocol for FFPE sections on the Benchmark XT. Be advised that the
times will be approximate for you and that we use Dako P504s @ 1:50. Also, you
can call Ventana and request a rep. to come to your site and help you optimize
the stain.
1. Create/manage protocols
2.
Just another idea : I have used dental wax. Comes in a package of about
20 sheets pf pink wax, each 7.5 cm x 14.5 cm x 0.2 cm. It's a little bit
more pliable than the waste paraffin, but not as cheap :-) I cut it in
a long rectangle so that it sticks out of the formalin with the tissue
at the
Please Send Resume for:
Part Time opportunity for a Histotechnican.
Duties and responsibilities include:
• Under general supervision performs routine and non-routine activities
involved in the preparation of slides, for microscopic evaluation by
pathologist(s) according to policies and
Bonjour,
Je suis absent du laboratoire jusqu'au jeudi 03 mai 2012. Je vous répondrai le
plus rapidement possible.
Eric Tambutté
Thank you for your mail. I will be out of office till May 03rd 2012.
I will respond to your e-mail as soon as possible.
Thank you for your understanding.
Best
About pinning specimens so they fix flat: Margaret Horne notes the use
of dental wax. I've used it to make small boats for the rather
exacting procedure of pinning muscle biopsy specimens for electron
microscopy. Here a 1 to 2 mm bundle of longitudinal fibers has to be
gently stretched to its
Dear All,
Can some one please send the protocol for immunofluorescence, I want to stain
for FITC CD45.1 on mice spleens.
Thank you very much for your help.
Many Thanks,
Anil kumar.
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The way we got around that problem was to do a manual PAS stain instead!
Josie Britton HT
Cheshire Medical Center
Keene NH
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott,
Allison D
Sent: Thursday,
Hello all,
I am new to your listserve. I am wondering if anyone has experience with the
Leica Peloris processor. Specifically, has anyone noticed if the higher
processing temperatures has affected any IHC staining results or FISH results.
Looking forward to responses,
Joe W. Walker, Jr.
In my experience, no. Our current IHC/ISH is as good, if not better than what
we were getting with our former processors.
Richard
Richard W. Cartun, MS, PhD
Director, Histology Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
We have 4 peloris processors, run many IHC and FISH stains, none of them
have had any negative effects due to the increased temperatures. The
increased temps aren't that high, 45 degrees for all the liquids (we use
xylene) and 65 degrees for the paraffins.
Good luck
Patrick
On Fri, Apr 20,
Hi,
Strange issue with your Ki-67. I don't know what would do that unless
it was non-specific background. Is it nuclear or cutoplasmic?
If you are working in mice or other animals you could try labeling
them with BRDu then detecting it with an anti BRDu antibody. Humans don't
like being
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