[Histonet] DFA Method for the diagnosis of PCP on sputa.

2012-06-02 Thread tahseen
Dear All, Good Morning, We are going to start DFA Method for the diagnosis of Pneumocystis carinii pneumonitis from Sputa or Tracheal Aspirates from Humans. Would anyone like to share a method/protocol for DFA? Thanks and good weekend to all Muhammad Tahseen MLT (Punjab medical Faculty) MT

Re: [Histonet] In FL, who can perform IHC/ISH?

2012-06-02 Thread Jay Lundgren
I had an assignment in SW Florida not too long ago, doing mostly IHC, and at that time I was told that FL requires a tech doing IHC to be a Florida registered Technologist. (Florida has three levels of registration, technician, technologist, and supervisor.) *NOTA BENE*: This is NOT the

Re: [Histonet] DFA Method for the diagnosis of PCP on sputa.

2012-06-02 Thread Rene J Buesa
Use Grocott's methenamine silver method. It has become the standard procedure for P.carinii René J. --- On Sat, 6/2/12, tahs...@brain.net.pk tahs...@brain.net.pk wrote: From: tahs...@brain.net.pk tahs...@brain.net.pk Subject: [Histonet] DFA Method for the diagnosis of PCP on sputa. To:

Re: [Histonet] Stains

2012-06-02 Thread Rene J Buesa
Use modified Steiner with phosphotungstic acid instead of uranyl nitrate. René J. --- On Fri, 6/1/12, Dianne E. Holmes dhol...@umc.edu wrote: From: Dianne E. Holmes dhol...@umc.edu Subject: [Histonet] Stains To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Friday,

Re: [Histonet] In FL, who can perform IHC/ISH?

2012-06-02 Thread Kim Donadio
This is correct. The only exclusion is a private office lab only needs to meet CLIA sub part M requirements. The science courses basically CLIA has been lenient on this but are now getting tuffer with the new healthcare regulations coming out. I don't want to make this a threat but everyone

Re: [Histonet] In FL, who can perform IHC/ISH?

2012-06-02 Thread Kim Donadio
All sorry about my double posting. My iPhone must have an echo. :( Enjoy life! Sent from my iPhone On Jun 2, 2012, at 1:11 PM, Kim Donadio one_angel_sec...@yahoo.com wrote: This is correct. The only exclusion is a private office lab only needs to meet CLIA sub part M requirements. The

[Histonet] Pinkus' acid orcein Giemsa stain

2012-06-02 Thread Bob Richmond
Luis Chiriboga (at NYU) asks for a method for Pinkus' acid orcein Giemsa stain. I haven't performed this stain or seen it done, but I copied the method out of Pinkus' book quite a few years ago. I don't know if anybody is still doing this stain. Orcein is a natural dye (from a species of lichen)

[Histonet] BLock alignment tool

2012-06-02 Thread Amos Brooks
Hi, There is one on every microtome made. The two little knobs align the block just perfectly to any angle previously cut. It is much better than just whacking into a block that was cut slightly differently than what a dumb instrument is telling you is aligned. Knowing how to use this

Re: [Histonet] BLock alignment tool

2012-06-02 Thread Kim Donadio
I thought a alignment tool was one of those weird things histotechs used to make sure their toenails were even on all 5 toes ? Dang! Sent from my iPhone On Jun 2, 2012, at 2:31 PM, Amos Brooks amosbro...@gmail.com wrote: Hi, There is one on every microtome made. The two little knobs

[Histonet] Retic Stains

2012-06-02 Thread Robert L. Lott
The Gordon and Sweets, Gomori, Laidlaw, and Nasher and Shanklin methods for reticulin do NOT use Uranyl nitrate as a sensitizer. The Snook and Wilder methods do. Robert L. Lott, HTL(ASCP) From: Dianne E. Holmes dhol...@umc.edu Subject: [Histonet] Stains To: