RE: [Histonet] cytology control slides?

2012-06-13 Thread Edwards, Richard E.
???buccal.. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: 11 June 2012 15:48 To: pathr...@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cytology

[Histonet] RE: processors

2012-06-13 Thread Tom McNemar
VIP all the way. Still got one that I bought in 1985 as a backup Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] OSU Job Posting

2012-06-13 Thread Thorn, Lindsay
The Wexner Medical Center at The Ohio State University is looking for two (2) full time Histology Technicians/Technologists. The Ohio State is located in the heart of Columbus, Ohio. We have four beautiful seasons a year and enjoy a reasonable cost of living. Interested candidates should

[Histonet] HTL needed - FT Days--flexible hours

2012-06-13 Thread Cheryl
Small community hospital with a nice family feel (great schools/parks/cost of living) is seeking a registered HTL with a BS in one of the sciences.  The conversation is simple and you can talk with the facility folks without any committment...we're looking for the perfect fit for the hospital

[Histonet] Temp travelers needed

2012-06-13 Thread Cheryl
Hi Guys--   We're getting busy again and need a few more travelers.    ---If you're curious, we'll spend some time answering your questions.    ---If you're ready, we have a couple of short openings to talk about.    Either way the conversation is fun and informative--the good, the bad, the

Re: [Histonet] Processing issue

2012-06-13 Thread Rene J Buesa
That will depend on the tissue and the size/thickness of the specimens. Appreciate how they turned out and you will have the answer to your question. At least for me it is very difficult to answer your question without knowing the tissue characteristics. René J.

[Histonet] Antigen retrieval

2012-06-13 Thread Mike Tighe
Does anyone have a favorite antigen retrieval method for FFPE mouse tissues that they would be willing to share? I have been using citrate buffer Ph6.0 with poor to moderate results. Thanks for any help! Mike ___ Histonet mailing list

[Histonet] RE: Antigen retrieval

2012-06-13 Thread Elizabeth Chlipala
Mike Retrieval methods are also based upon the antibody. During protocol development we try several different retrieval methods - no retrieval, enzymatic (proteinase K), pH6 and pH9, we then determine the best method and go from there. One retrieval method may not work for all antibodies.

[Histonet] RE: Antigen retrieval

2012-06-13 Thread Goins, Tresa
Mike, A good retrieval method will restore the epitope to a configuration that the antibody will recognize. The data sheet received with a commercial antibody may suggest a retrieval method to use, but if you are using a non-commercial source, trying a variety of methods may help unless the

RE: [Histonet] cytology control slides?

2012-06-13 Thread Yang, Mari
I don't think there is a requirement for control slides for Cytology, only when IHCs and special stains are required. CAP requires a mechanism for monitoring cross-contamination. For urine cytology, I would insert a blank slide with your staining rack, if you are batch staining to ensure there

[Histonet] job opening

2012-06-13 Thread Hutton, Allison
We currently have an open position for a HT, full time days. We are a community hospital located in southeastern PA. We average 11,000 cases per year, about half of which are GI biopsies. For more information or if interested, please visit www.dh.org

RE: [Histonet] RE: Antigen retrieval

2012-06-13 Thread pruegg
The most important part= about working up a new antibody is to have known positive tissue to use as= a control, if you do not have that everything you do is a shot in the dark= , you will not know if your protocol is not working or the control you are = using is not

[Histonet] RE: Sequenza units for manual immunohistochemistry (Keri Colwell)

2012-06-13 Thread Liebig, Tyler K.
Hello, This is Tyler Liebig with the Thermo Scientific IHC group. I'm responding to Keri's questions regarding the Sequenza manual staining. Keri you are correct that if the waste is not emptied and builds up too much in the bottom of the sequenza unit it will definitely stop the capillary

[Histonet] RELIA Hot Hot Hot Histology Job Alert!! Please take a look at some exciting opportunities.

2012-06-13 Thread Pam Barker
Hello Histonetters! I hope everyone is having a great day and keeping an eye out for armadillos!! :-) I have some new positions that I am excited to share. I am excited about these opportunities because some are with brand new labs, some are exclusive to RELIA and some are just really really good

[Histonet] RE: Varistain 24-4 Stainer Manual

2012-06-13 Thread Burton, Lynn
I have an operator guide. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D [allison_sc...@hchd.tmc.edu] Sent:

[Histonet] TEM used needed

2012-06-13 Thread Abdullah Thayyil
Hi I am looking to start a TEM lab in India. I heard that used TEMs are available if one is willing to pay for shipment. Can someone give some information? Thanks Abdullah ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Histology Opportunity Northeast (NJ/PA/NY/MA)

2012-06-13 Thread Matt Ward
Good Afternoon Everyone! We are searching for a histologist who is looking for an exciting career with a world leader in manufacturing histology products. Our client is looking for a Applications Support Specialist to be the Histology expert in the Northeast to provide customer and sales