Hi Histonetters!
I hope everybody is having a great day. I have a few new jobs to share with
you as the phone is ringing off the hook and the list of opportunities keeps
growing and growing. All of these jobs are permanent full time positions
with excellent pay and great benefits. The clients I
Is it possible to perform an H&E stain on tissues embedded in Epon plastic
and cut into 0.5 - 1.0 micron sections? I can find protocols for doing so
with tissues embedded in methacrylate, but not in Epon. Would the protocol
for methacrylate also work for Epon, and if not, why? I am a student
workin
Hello Sarah and Histonet
You get wonderful formalin fixation of all rat tissues by transcardial
perfusion - and no blood cells. You don't say whether you need a specific
muscle and if it needs to be from a specific rat.
Before you get into the bother of perfusing (& IACUC approval) you might w
Thank you Dr. Wright!
We have full IACUC approval for perfusion and for all our procedures, and we
have done a lot of paraformaldehyde perfusion fixation. It is just that for
this study, we would like to avoid it, since it might interfere with other
aspects of the experiment.
So, we were wonder
Congrats on passing! What can I expect when I take it? Was it horrible? Kaiser
regional in Berkeley are always hiring, usually night shift. They are union.
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The following is the response I recived from a coding specialist at the
American Academy of Dermatology. I am trying not to be concerned that the
reference is 6 years old but I think it clears up what we thought to be true.
88342 for IHC
88314 other “special stains”
Here is the description
Mohs or not 88314 is for a special stain. They call this histo chemical
because chemical reactions take place in the tissue. This is not IHC. If
you want to bill for IHC on frozen sections you bill 88342. I just lloked
this up in my trusty coding guide.
Hope that helps or adds to the controversy as
Great team work! Job well done and a absolute answer is given.
Thank you
From: Carol Torrence
To: 'Kim Donadio'
Cc: "'Weems, Joyce K.'" ; 'Ingles Claire'
; histonet@lists.utsouthwestern.edu
Sent: Tuesday, June 19, 2012 2:10 PM
Subject: RE: [Histonet] Bi
Hi all,
I would like to stain for macrophages in mice aorta (5um frozen
sections). Would you have suggestions on which stains to use and perhaps
share the protocol?
Thanks in advance,
Daniela Bodemer
Research Assistant
Surgical Research, Infection and Immunity
Murdoch Childrens R
Well, I don't know if that settles that.
I haven't responded, because I have not worked for a Mohs dermatopahtologist
who runs Immunos (I have worked at numerous Mohs laboratories), however, this
explanation is contradictory. "Each stain is reported only once per block, not
per slide or per la
Serotec has two antibodies that will work a F4/80 and CD68, both are rat
anti-mouse. We have used them in the same application that you will be using
(mouse aorta) with good success.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3
Hi. all
Let me add another question linked to marcophage staining.
So far we have done macrophage staining several times with F4/80, and
in most of the cases
IHC images were quite beautiful, for liver, spleen, adipose tissue and so on.
BUT, we have not succeeded in staining lung alveolar macrophage
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