Our Prayers have been answered!!!
Richard Cartun rcar...@harthosp.org 7/17/2012 7:04 PM
Most of you know that I have been advocating for the elimination of the
Negative Reagent Control when using a non-avidin-biotin detection system
(polymer) in immunohistochemical testing. In my opinion, it
What wonderful news
Martha Ward, MT (ASCP) QIHC
Manager
Molecular Diagnostics Lab
Medical Center Boulevard \ Winston-Salem, NC 27157
p 336.716.2109 \ f 336.716.5890
mw...@wakehealth.edu
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Does anyone on the net do or know of a lab that does IL-5 IHC for
clinical/diagnostic purposes?
TIA
Luis Chiriboga Ph.D, Director
OCS Experimental Pathology IHC Core Lab
NYU School of Medicine
Medical Science Building Room 124
646-501-6934
luis.chirib...@nyumc.org
Outstanding!!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun
Sent: Tuesday, July 17, 2012 7:05 PM
To: Histonet
Subject: [Histonet] Negative Reagent Control
Most of you know that I have
I'm wondering if anyone has recommendations for CD4 and CD8 antibodies for FFPE
mouse tissue. Looking through the archives I see there was only success on
frozen tissues.
Has anything changed recently?? Or should I stick with CD3.
Thanks,
Brett
Brett M. Connolly, Ph.D.
Principal Scientist,
I work in a small lab (office bldg) that has been turned into histolab. We
have a portable work station with a vent to out side. (roof) I also have my
VIP processor in this room which has the filter carbon (activated) on the
processor. Where we have an issue is , on very windy days there
Hi Histonetters!!
I hope everyone is having a great day. Are you keeping cool on these hot
summer days?
Summertime in the South is Sizzlin hot and it's not just the temps!!
I have some great new opportunities to tell you about:
I have full time permanent positions with some of the best employers
I am flying into Bellingham, WA on Friday Sept. 28th to attend the NSH S/C
(much cheaper than flying into Vancouver) at 2:38pm and am looking to share
a limo to Vancouver with 5 or 6 people. If you are interested please email
me. If I get enough interest, I will follow up and see if that is
Hi Ateret,
You've got a few choices, some of which are free and some of which are
quite expensive.
In many soft tissue staining protocols, adipocytes show up as white, oval,
void spaces. Which is good!
Software like ImageJ http://rsbweb.nih.gov/ij/, or its more integrated
variant FIJI
Just found out I have to go to a meeting in 2 hours for the rest of the day (I
haven't even eaten lunch yet). I do IHC manually, they just got done cooling
off from HIER...I can store them overnight in buffer right?
Just double checking myself as this run is an antibody validation run...
-S-
Hi,
That is a pretty common kind of measurement.
You will need a digital camera attached to your microscope, and a stage
micrometer. You can then use the free software Image-J to get area
measurements. If you have some extra money Image-Pro software from Media
Cybernetics is the deluxe
I am wondering if anyone out there is using black biopsy paper to give to
offices to place the biopsy on prior to putting it into formalin? It looks kind
of like construction paper. If you are using this can you please give me a list
of pros and cons associated with it?
Thanks,
Becky
I vote No - this run would be valid only if you held all future slides
overnight after HIER.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart
Sent: Wednesday, July 18, 2012 11:44 AM
To:
Becky,
We have used this paper. What I found is that when submersed in the formalin
the black dye leaches out of the paper and the portion of the tissue touching
the paper turned somewhat black. These were tissues left on the paper and in
formalin overnight. Since I never processed that
I agree
On Jul 18, 2012, at 2:35 PM, Goins, Tresa tgo...@mt.gov wrote:
I vote No - this run would be valid only if you held all future slides
overnight after HIER.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
But only because it's a validation run.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Erin and Jesse
Sent: Wednesday, July 18, 2012 3:35 PM
To: Goins, Tresa
Cc: histonet
Subject: Re: [Histonet] RE: Hold
No you can't do that. If it were just a research IHC then you could give it a
whirl, write down what you did and often it would work just fine depending on
antigen and antibody.
But if you are validating an antibody for clinical lab you have to document you
follow GLP SOP every time else you
Hi All,
I will be trying out new H+E autostainers and cover slippers soon. Does
anyone have a favorite, and why? Thanks for your responses.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
Leica Autostainer XL with CV5030 coverslipper and transfer station.
This has been a real timesaver for us. It automatically moves the
slides from the stainer to the coverslipper. It has been relatively
trouble free.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
We have a Sakura Tissue Tek Prisma and Glas coverslipper we really like it.
It's a workhorse.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com
Ship
I second on that!
Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
It is added to formaldehyde to prevent polymerization of the formaldehyde.
Bader Siddiki bade...@gmail.com
Sent by: histonet-boun...@lists.utsouthwestern.edu
07/17/2012 01:44 PM
To
Rene J Buesa rjbu...@yahoo.com
cc
histonet Histonet@lists.utsouthwestern.edu, Kelly Boyd
kdboydhi...@yahoo.com
Sakura Prisma w/ tape cover slip. It also is available w/ glass cover slip.
Reliable, fast and a workhorse.
William DeSalvo, B.S., HTL(ASCP)
From: bdebrosse-se...@isisph.com
To: sandra.harris...@va.gov; har...@oncology.wisc.edu;
histonet@lists.utsouthwestern.edu
Date: Wed, 18 Jul 2012
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