Hi all...
Had someone in here who is interested in sectioning old bones - any ideas
as to what would be the best embedding medium? These are bones from a
graveyard more than 100 years old - not fossils.
Decal is out of the question - so it would have to be a resin of some sort.
Tips, ideas,
I understand the point about the biotin and I should have said that when
using the ABC method we have taken to always using an avidin/biotin
blocking kit. We are using biotinylated secondary antibodies from Vector. I
have seen the same problem occur in our anti-mouse, anti-rabbit and
anti-goat. In
Are you getting false positives and variations on the same control tissue for
different days ?
Sent from my iPhone
On Jul 24, 2012, at 8:13 AM, Eva Permaul e...@georgetown.edu wrote:
I understand the point about the biotin and I should have said that when
using the ABC method we have taken
We standard use a Citrate pH6. We do 20min at 98C followed by cooling in
the citrate for 20min.
Eva
On Tue, Jul 24, 2012 at 8:29 AM, James Burchette Jr.
james.burche...@duke.edu wrote:
What is your heat retrieval process?
Jim Burchette, HT(ASCP) QIHC
Histologist and Fly Fishing Bum
These samples were all human but I have seen it in Mouse mammary gland as
well but those nuclei were lighter.
Eva
On Tue, Jul 24, 2012 at 8:51 AM, James Burchette Jr.
james.burche...@duke.edu wrote:
Thanks Eva. I don't know why you are having the nuclear staining problem.
Your retrieval
Hello Histonetters,
Has anyone done IHC using Cyclin D1 antibody? What vendor was used? What
control tissue was used?
Thank you.
Donna Suresch - Merck Co.
Donna L. Suresch
Imaging Research Scientist
Merck Research Laboratories
Department of Imaging - West Point Campus
Mail Stop: WP44K
We have used Neomarkers/Labvision cat.no. RM-9104 on mouse salivary glands
and mammary glands.
On Tue, Jul 24, 2012 at 11:16 AM, Suresch, Donna L. donna_sure...@merck.com
wrote:
Hello Histonetters,
Has anyone done IHC using Cyclin D1 antibody? What vendor was used? What
control tissue was
Hi Donna,
We use Dako #M3635 at 1:100. Mantle cell lymphoma is our control.
Mark
On Tue, Jul 24, 2012 at 8:16 AM, Suresch, Donna L.
donna_sure...@merck.comwrote:
Hello Histonetters,
Has anyone done IHC using Cyclin D1 antibody? What vendor was used? What
control tissue was used?
Thank
Please remove me from your list.
Thanks,
Stacey Crenshaw
Human Resources Generalist
Oregon Medical Group
1580 Valley River Drive, Suite 160
Eugene, OR 97401
Phone (541) 242-4339
Fax (541) 284-2038
Disclaimer: This electronic message may contain information that
Can I store (Xylene substitute, Isopropanol and Formalin) each, separated in 5
gallons (closed with a tight lid) plastic containers placed inside spill tray
in a room that the temperature can rise up to 40°C in the summer? One container
at the time, before it gets pick up by waste company.
Hi Everyone - This past HistoTALK http://www.histotalk.com/ show on Sunday,
July 22nd had as its guest Wanda Jones from Emory University Hospital. Just
wanted you all to know. Oh, and BTW, before that show #28, our guest was Billie
Swisher! Two wonderful interviews.
Yours,
Dave
OH NO! They're BACK! Saints preserve us!
- Original Message -
From: Stacey Crenshaw screns...@oregonmed.net
To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu
Sent: Tuesday, July 24, 2012 9:51:37 AM
Subject: [Histonet] Unsubscribe
Please remove me from your
I would be interested in this as well...
Thank-you,
Glen Dawson BS, HT(ASCP), QIHC
Histology Technical Specialist
Janesville, WI
From: 41dm...@gmail.com
Date: Fri, 20 Jul 2012 14:03:36 -0400
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CD200
Is anyone out there running
So, I go on maternity leave in 52 (I hope...) days...while out I will have one
of those automatic messages that says I'm out...Since I get say 20ish emails a
day from histonet I would assume that 20ish of these replys will go out to
everyone, and I don't want to drive people bonkers.
Maybe the
Unsubscribe, please
This message (including any attachments) may contain confidential, proprietary,
privileged and/or private information. The information is intended to be for
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intended
Hi Sarah,
I had a similar situation. If you go into your profile on the Histonet site
you can click to not receive any of the Histonet messages while you are
away. Then when you get back and want the messages again you just change it
back.
Eva
On Tue, Jul 24, 2012 at 2:35 PM, Sarah Dysart
Yes. The strength of the stained nuclei in the no primary slides are
stronger on some days than others.
On Tue, Jul 24, 2012 at 8:18 AM, Kim Donadio one_angel_sec...@yahoo.comwrote:
Are you getting false positives and variations on the same control tissue
for different days ?
Sent from my
Hi Everyone:
I had a few questions regarding Bielschowsky silver stains.
(1) What adhesive (if any) or type of slide do you use for the stain?
(2) How do you clean the glassware?
(3) When diluting the 40% formaldehyde when making up the developer, do
you consider the 40% formaldehyde as 100%
Okay 30 years is too long in histology! I should have retired sooner than I
did,. I saw this subject heading as a new stain for bacteria instead of an
ad for a bed and breakfast in Tennessee.
Rena Fail
___
Histonet mailing list
Hello!
My name is Jack Ratliff and I am Chairman of the Hard Tissue Committee (HTC)
for the National Society for Histotechnology (NSH). On behalf of the HTC and
the NSH, we would like to invite you to attend and participate in the 2012 Hard
Tissue Forum. This event will take place August
Description
The Tri-Institutional Laboratory of Comparative Pathology provides research
pathology support to investigators at Weill Cornell Medical College, Memorial
Sloan-Kettering Cancer Center, and The Rockefeller University, located in New
York City. This position supports the mission of
Change all your reagents.
Change periodic acid every week at least.
Personally I see little advantage in fast green, and a common downside is
overstaining in many labs, hiding fungus and basement membrane.
Faint Hematoxylin is acceptable counterstain.
For certain applications, e.g., dual
I vote for Raspberry!
Claire
From: histonet-boun...@lists.utsouthwestern.edu on behalf of nmhi...@comcast.net
Sent: Tue 7/24/2012 11:36 AM
To: Stacey Crenshaw
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Unsubscribe
OH NO! They're BACK!
Hello Eva
I had a similar problem with an actin antibody many years ago. We eventually
after many trials decided that it was an artifact caused by the heat retrieval
possibly in relation to the length of fixation time. By playing with the
dilution we eventually eliminated the problem by not
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