AW: [Histonet] Unsubscribe

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[Histonet] old bones

Thanks all for suggestions ... as always you guys rock! Sadly, this may almost be my last post - after close to 30 years in the lab, I'm moving into another arena all together - environmental health! So this may well be farewell to all you great guys and gals who have provided help and advice

[Histonet] Markers for Rat Samples

Hi All, I am in search of an IF marker to use in the detection of rat natural killer cells. From what I have found, I have seen only antibodies that were made using rat tissue which would not work for us. Any suggestions? We are also looking for markers for rat macrophage, rat B-cells, and rat

RE: [Histonet] Bielschowsky questions, Luxol Fast Blue answers, and Protocol Drift.

Hi Tim, In our lab, we use positive charged slides for all special stains. We clean our glassware using a low-sudsing soap with bleach (~1 cup) added and then rinsed 3 times using distilled water. When we make up the formaldehyde solution, we consider the 40% to be 100% in making the dilution.

[Histonet] GMS stain on blood smear

I have an aspirate from a mass on an animal. It was very bloody, I have spun down a portion of the sample. Does it need to be fixed with alcohol before I make slide smears? I need to do a GMS on a direct smear. What are the staining steps I need to do for these smears? Any info would be

[Histonet] Decalcifying bone

In looking through old procedures, I have found several different 'solutions' that have been used for fixation and decalcification of bone (particularly bone marrow cores).  I would very much appreciate hearing what people are using today for optimal fixation and decalcification of bone for

Re: [Histonet] RE: Secondary antibody causing nuclear staining

I do see positive nuclei in the NC. That is what I am asking about. I know I could switch methods but my question is also why if it is happening is it not as strong all the time? Why are the cells very light one day and dark the next? What is causing them to stain? Just curious is all. Eva On

[Histonet] Thank you for coverslipper responses

Thank you everyone for your input on the different types of coverslippers.  I'll be sharing the information with the others that I work with so we can make a decision.  I'll be happy to share my thoughts after using whichever we choose for a while. Brendal C. Finlay, HT (ASCP) West Florida

[Histonet] CAP Sample Exchange Registry

All, I am the current chair of the CAP's Immunohistochemistry Committee. One of the things we've been grappling with is how to help IHC laboratories evaluate difficult-to-validate antibodies (e.g. HSV, spirochetes, etc.). Some years ago, our molecular pathology colleagues at the CAP

[Histonet] Re: Markers for Rat Samples

You could have a look here, in the image gallery- top left link under Immunhistochemistry . Then scroll down to individual protein/ab hyperlinks. Maybe you will find some suitable Abs. NB: Sure you can use rat primaries on rat tissue, it just depends on whether you are looking for NK cells

[Histonet] Thanks for all the good wishes

AlsoI'll try to get the UNSUSCIBE UNSCRIBE UNSUSCRIBE thingy right when the time comes! -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) A good head and a good heart are always a

RE: [Histonet] Decalcifying bone

For fixation, we use Z-fix from Anatech, LTD. It gets the great sharp nuclear detail that's so imperative to diagnosis but without the environmental issues of the old B-5 fixative. After fixation, we decalcify in a formic acid solution called Immunocal from Decal Chemical Corp. Our IHC works

[Histonet] Re: Decalcifying bone

Jeanne Clark, Histology/IHC, Stanford Hospital and Clinics asks: In looking through old procedures, I have found several different 'solutions' that have been used for fixation and decalcification of bone (particularly bone marrow cores). I would very much appreciate hearing what people are

[Histonet] Rabbit M1 and M2 Markers

What are people using to differentiate between M1 and M2 cells in rabbit tissues?? Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination,

[Histonet] RE: Decalcifying bone

Ms. Clark We are currently use 10% formic Acid in a microwave oven to decal ours for about 2 and 1/2 hour after they were fix in 10% neutral buffer Formalin. We were told that they are Ok for immuno-stains. Thomas Huynh, HT( ASCP) Clinical Histology Technician Pathology/ Histology/ Bone Lab

Re: [Histonet] Bielschowsky questions, Luxol Fast Blue answers, and Protocol Drift.

Hi Tim, It is safe to consider 40% formaldehyde to be a 100% solution, because that is the maximum amount of formaldehyde that will dissolve into an aqueous solution. Happy Fixation, -Eric Eades Phenopath Laboratories Seattle, WA On Tue, Jul 24, 2012 at 12:36 PM, Tim Wheelock