Sorry I don't not have more info on the DAPI user. Unfortunately...or rather
fortunatelyI have never personally had this problem. This is a corporate
user, we have done some H&E's for, on FS with no issues. This is just not a
problem I have ever had. I will pass these on to him; they are int
I used the Peloris at Labcorp for about 2 years. I liked it a lot. It tells
you when the reagents are ready to be changed, and can do two separate runs
simultaneously. Like any machine it has drawbacks such as reagent competition
and no it doesn't tell you how to correct a problem. One time
When you use "smear", do you mean that the nuclei are blurring/ill-defined ,
with threads of DAPI-positivity coming away from the nuclei?
If so, it is due to underfixation.
Acetone is not a proper fixative.
In the time one uses is, it rapidly delipidises the tissue, rendering it better
preserved,
Carol wrote:
Someone sent me a question regarding DAPI staining ( I generally use
Hoechst )...but anyway, they are having smearing of nuclear contents. I
would guess that is from rupturing of the nuclear membraneover fixation?
Over air drying? They are fixing 10 mins in cold acetone and
Hi Angela
Our lab has repeatedly had problems with the Leica Peloris!
Fortunately (for Leica) the Peloris processes tissues very well, so our
lab has persisted with our two machines for around 5 years now.
I believe there is now a "version 2" machine on the market that corrects
many of the n
