Cooling on ice for 10-15 min renders the block cool enough for trimming and
consecutive cutting without the need of a freezing spray.
We use cooling devices at -15 degrees. They have usually a nice
snow-surface, that gives the block some moisture during cooling. Especially
blocks, that have to be
Jenny My experience and training is to use some method involving ice or at the
very least a cold-retaining tray made to chill blocks. I also was taught this
method in histology school , in clinical training at four quite large
institutions, and also have used some variation of an ice cooling
It has been my experience that using freezing spray will cause artifacts in the
paraffin block as well as the tissue. We are a high-throughput lab where all
the techs face all their blocks then put them on a block of wet ice prior to
microtomy. I am not a fan of freeze sprays, personally.
Jenny:
Had it been based on technique, you should be the supervisor.
Let me go step by step:
1- we always used those gelatin filled trays that are frozen and from the
productivity and quality point of views, it is better to trim all the
blocks one tray first and place them back face down to
Hmmm
Nobody has yet mentioned the rationale behind the need to cool Pwax blocks.
Sure, it makes sectioning easier butnot always.
Never forget the huff!
Let's get back down to microtomy basics, so newbies have an appreciation that
sectioning is a mechanical process, as well as an
I have a question in regard to eliminating the use of negative controls when
using a non-avidin-biotin detection system.
Do you not feel that negative controls may still need to be run in tissues
which are likely to be pigmented such as lymph node, skin and liver?
We were thinking to
I find that there are several negative effects from freeze spray such as
artifacts, safety concerns, cost consideration, etc. I have always used the
method you are were taught and my staff uses the same. You can trim the block,
cool on ice for 5 minutes then cut the final sections and still
Thanks everybody for your answers. I cant respond them all but I concluded
that the best way to get good sections is too chill the blocks on ice
because I agree that it facilitates the process.
I really don't understand why my supervisor depends so much on freezing
sprays to cut and the
JennyYou don't need to respond to this, but I will post in case there is anyone
else out there who is going through the same experiences and feeling
discouraged. There are many people in the field like this. I have been out
there at least a little while and I went through the same response
We have eliminated them all per the CAP guidelines.
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
This e-mail, including any attachments is the property of Saint
Carl
I think what you are referring to is a mention about the practical aspect of
providing support (from the microcrystalline structure of the wax) to the
tissue during sectioning against shearing effectsand I will add the
comment that the physical properties and performance of the
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