RE: [Histonet] Cooling paraffin blocks with ice VS. Freezing Spray

2012-10-02 Thread Susan.Walzer
Ice is still the best coolant because it adds moisture. As Lee Luna used to say Rehydrate, rehydrate, rehydrate For tough blocks, face it then add soapy water to ice with some ammonium hydroxide. Like magic! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] Ventana temp veriifer slides

2012-10-02 Thread Kuhnla, Melissa
We have been running in every position every quarter!! Very expensive. We record the lot # of verifier slides and the results. If one position fails, we repeat. No photos. We recently took advantage of Ventanas special promotion. They will come here and have someone perform all quarterly

RE: [Histonet] duel stains

2012-10-02 Thread McMahon, Loralee A
No we are still doing the triple stain, just stopped billing three. The pathologist decided that the patient specimen was more valuable. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210

[Histonet] MIXED fiber type identification by Immunohistochemistry

2012-10-02 Thread arjun ruhella
Greetings to all, In immunohistochemistry, I am encountering confusion in designating muscle fibers (based on staining color intensity) to a particular fiber type. This problem is particularly bothersome when i encounter the fibers stained with 2 different colors, one color has good

RE: [Histonet] duel stains

2012-10-02 Thread Sebree Linda A
We've always just billed for one stain so no difference for us; we'll continue doing it. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of llhartma...@comcast.net Sent: Monday, October 01, 2012 6:25 PM

Re: [Histonet] Acid Phosphatase Reaction

2012-10-02 Thread Rene J Buesa
Acid phosphatase is a standard procedure in FFPE (Formalin Fixed Paraffin Embedded) tissues. You will need to process your tissues accordingly. Xylene is also standard procedure even when it should be avoided because of toxicity. There are many other alternatives to xylene usage. I advise you to

[Histonet] Re: Reliable Histology Team Member to embed and cut for you.

2012-10-02 Thread Jay Lundgren
I am not the only forum member who is concerned about this. I have received private messages from others who agree with me and choose not to reply to all. I will not repost them out of respect for their privacy. The facts that you take what you do seriously, or are not disrespectful are moot.

[Histonet] Cold room alternative

2012-10-02 Thread Johnson, Aaron
The building I am in does not have a cold room and I would like to incubate some samples overnight on an orbital shaker at 4°C. Can anyone recommend a refrigerator with an interior electrical outlet or another alternative? Thanks. Aaron Aaron M. Johnson Assistant Professor Department of Speech

[Histonet] Traveling

2012-10-02 Thread Demetria Ross
I'm considering becoming a traveling histotech in the future. Are there any traveling techs or any techs that traveled in the past on the forum that can offer some ups and downs positive and negatives of traveling Thanks. ___ Histonet mailing list

[Histonet] RE: Cold room alternative

2012-10-02 Thread Bell, Pat
Aaron, We have a refrigerator from VWR that has a glass door like a Deli frig. and it has an electrical outlet inside as well as outside. That could be a starting place to look. :) Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. RC1-S, L18-8402C

[Histonet] Re:

2012-10-02 Thread Sadushe Loxha
http://ifet.com.ar/www.itrustyou.foxnews.realwork.php ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Cold room alternative

2012-10-02 Thread Connolly, Brett M
Ditto here, ours are manufactured by True, but you could probably find one cheaper through one of the used lab equipment vendors on the web. Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T-

Re: [Histonet] Traveling

2012-10-02 Thread E. Wayne Johnson
If you do that and announce your availability on Histonet, you are likely to be criticized publicly by a few, while others send email and twitters to one another privately discussing your attributes, and those who like having the information that you are available probably will not speak up in

Re: [Histonet] Reliable Histology Team Member to embed and cut for you.

2012-10-02 Thread Marc DeCarlo
Go get em Jay! I agree with you 100% this forum should be kept free of blatant advertising. I'd also like to give you credit for not stooping to the level of this anonymous poster after he or she publicly tried to belittle you. Marc DeCarlo On Tuesday, October 2, 2012, Jay Lundgren wrote: I am

[Histonet] Reliable Histology Team Member to embed and cut for you.

2012-10-02 Thread Contact HistoCare
Ok, let me put this issue to bed. It's fine you feel you need to be the voice for those who have all the help they need and will never need the services of a histology professional to help cover staffing shortages or inadequate/ineffective staffing. There are thousands of subscribers to this

Re: [Histonet] Reliable Histology Team Member to embed and cut for you.

2012-10-02 Thread Contact HistoCare
Kudos to you too for reading the entire message. If you had, you'd see the other person initiated the public belittling. I am not some pushover who won't defend my professionalism when subtly attacked by some closed minded person and his cheerleaders. I didn't ask for this. It was brought to