Some time back, I'd been in touch with an HT at (I think) Baptist Hospital in
Oklahoma City regarding training for a new histotech. I met this lady at the
Florida NSH meeting and we had been in touch for a time afterwards. I have a
candidate that needs to join a training program and I'd
Hi histonetters!
I'm just attending a histo-course, where the teacher told us his opinion
about overfixation.
For him overfixation takes place in any formaldehyde solution with a
concentration above 5%. This should cause the margin-artefact, that leads to
false-positive IHC at the margins of the
Hello, I was curious if anyone had experience with urology tissue? What are
some of the most popular special stains you performed? I'm trying to get more
familiar with these kinds of tissues. Is there any histotech website that had
helpful information for urology specimens?
I read one paper or abstract recently where sections were made at 50
microns then used
successfully for RT-PCR. I am pretty sure that it was PRRS virus that
they were pursuing.
On 3:59, Mark Tarango wrote:
I would try getting more sections from LCM and extracting into 7.5 uL, if
that
Gudrun
I would thank him for his opinion and move on.
There has always been the unfortunate tendency to accept anything that is
published or presented formally as if it were gospel.
I am a lot more skeptical, give me proof.
Barry
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