Re: [Histonet] chemical disposal

2012-11-29 Thread Lee Peggy Wenk
Where most people get confused is theBiological Hazard. They think - this chemical would hurt a human being, it would damage someone's skin if splashed on it, it would injure someone's lungs if inhaled, it could cause cancer with long exposure, etc. Since it's hurting a person, it must be a

[Histonet] RE: My earlier post about ASCP requirements in MI

2012-11-29 Thread Pam Barker
Hi Histonetters!! Thank you to all my histobuddies that responded about the certification in Michigan question. Of course I am going to encourage them to hire a certified tech it was a question the client asked that I needed to get answered and I appreciate you taking the time. Happy Holidays!!

Re: [Histonet] Validation of a new tissue processor

2012-11-29 Thread Rene J Buesa
Absolutely agree with Tim's comments. I would even add that if the new tissue processor uses the standard heat/agitation/vacuum/pressue sequences as the old one, the validation objective is mainly directed to have some documentation that the facility's attorneys can have in case something goes

[Histonet] Hii everybody

2012-11-29 Thread vaigunda ragavendran
Hii Histonetters, Happy holidays. I am on the lookout for a marker to be checked using immunohistochemistry that would get expressed at the DRG in response to streptozotocin-induced diabetes. I am basically looking for a neuronal marker that would give an idea of the degree of microvascular

[Histonet] Lung histology

2012-11-29 Thread zakky cholisoh
Hello lovely people! Is there anyone working with mouse lung (or any lung)? I work with respiratory virus in mice and now about to begin the fun part of the work, histopathology :) I was just wondering what is the best section of the lung, is it coronal section or tranversal (horizontal) section?

[Histonet] Radiatiation Safety

2012-11-29 Thread Deloris Carter
Hi all, I'm trying to complete a protocol for radioactive specimen handling. I need to know if anyone has suggestions for this. We do not use a geiger counter for specimens. The radiation safety person in nuclear med says their policy is that technicium 99 has such a short half life, and that

RE: [Histonet] Radiatiation Safety

2012-11-29 Thread Craven Peter (NHS HIGHLAND)
Deloris When we set this up we got our Radiation Protection Services to write a safe handling policy for the tissues (sentinel nodes) and added this into our Standard Operating Procedures. Might as well get the advice from the experts and trust it rather than making your own policy. Peter

[Histonet] cellblock help

2012-11-29 Thread Davis, Cassie
Good afternoon Histonet folks, I am hoping somebody might be able to help me with a protocol using Thrombin to get a cellblock from a specimen with scant material. This process was brought to my attention a few years ago but I never saw an actual protocol. Thanks for any help have an awesome

[Histonet] Re: Histonet Digest, Vol 108, Issue 37

2012-11-29 Thread Madeleine Huey
Message: 2 Date: Thu, 29 Nov 2012 16:46:03 + From: zakky cholisoh zakky...@gmail.com Subject: [Histonet] Lung histology To: histonet@lists.utsouthwestern.edu Message-ID: cak9ujsdf3coo1pw+cxgjl9m0bmz+wwyfz6l0o7r5m8kbqrq...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1

[Histonet] Re: Histonet Digest, Vol 108, Issue 34

2012-11-29 Thread Madeleine Huey
On Wed, Nov 28, 2012 at 7:10 AM, histonet-requ...@lists.utsouthwestern.edu wrote: From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Kim Merriam [kmerriam2...@yahoo.com] Sent: Wednesday, November 28, 2012 7:54 AM To: Histonet Subject:

[Histonet] Re: Lung histology

2012-11-29 Thread Erickson, Jamie E
HI Zakky, I've worked on a lot on mouse lung and the process of embedding can depend on what you are looking for or your pathologist opinion. In my OVA models I was looking at inflammation around the main stem bronchi and looking at goblet cell hyperplasia using PAS

[Histonet] Re: Radiation safety

2012-11-29 Thread Bob Richmond
Deloris Carter (where?) asks: I'm trying to complete a protocol for radioactive specimen handling. I need to know if anyone has suggestions for this. We do not use a geiger counter for specimens. The radiation safety person in nuclear med says their policy is that technicium 99 has such a

[Histonet] CRYOSTAT PROBLEM

2012-11-29 Thread Lakshmi Kammili
Hi all, We have a old cryostat (Microm h505 model). It was making some sudden vibrating noises for a little while and then smooths it self. There  are no temperature alterations every thing was fine but suddenly it stopped working, no display more looks like dead :(  I tried calling the service