RE: [Histonet] automated microtomes

2012-12-05 Thread Lynette Pavelich
I am purchasing my second Leica RM2255 automated microtome. Rene' is correct in saying that the carpal tunnel syndrome will eventually affect every advanced tech by using any microtome manually. On the RM2255, the flywheel additionally is automated. You can choose to use the flywheel manually

[Histonet] HistoTALK

2012-12-05 Thread David Kemler
Hello HistoNetters -   In case you missed our last two guests on HistoTALK http://www.histotalk.com/, they were Region VII Director, ANDI GRANTHAM and KATHERINE (Kaspar) KASPRZYK, President of the Arizona Society for Histotechnology. Just wanted to let you know.   Yours, David

RE: [Histonet] Tissue Processors

2012-12-05 Thread Marcum, Pamela A
We have 4 Thermo Excelsiors and prefer them to the VIP we no longer use. It is ease of changing reagents and programming as well as working better for our tissues. We also have Leica ASP300 that is used for only one type of tissue so we no longer need to change it weekly and expose personnel

RE: [Histonet] Tissue Processors

2012-12-05 Thread Boyd, Debbie M
Excelsior Great workhorse. Very little to no down time. Less tech time needed as reagents dump and rotate automatically. User friendly. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock

Re: [Histonet] Immunohistochemical detection of cytokines in older frozen sections

2012-12-05 Thread Rob Day
Folio biosciences is testing a process that will rejuvenate older sections to restore degraded antigenicity for ISH. It's designed to help scientists with exactly the problem you are describing Email me if you want to help us test this process using your slides. No charge of course. Rob Day.

[Histonet] RE: Histonet Digest, Vol 109, Issue 5

2012-12-05 Thread Riesen, Rebecca
I am in love with the Excelsior from the standpoint of hands off daily maintenance. Waste paraffin is pumped into a container with a liner, you let it solidify and remove waste in the bag for easy disposal. Pour a couple bags of paraffin pellets in and walk away. Take a bottle of waste

Re: [Histonet] Fixation time

2012-12-05 Thread Geoff
I agree with Rene. Geoff On 12/3/2012 12:00 AM, Daniela Bodemer wrote: Hi all, Our tissue processor has been shut down due to a contamination issue and now all the tissues (mice and rat pelves) collected prior to this happening have been sitting in 4% PFA. Some tissues more than a week,

[Histonet] Histology Positions in Little Rock AR

2012-12-05 Thread Marcum, Pamela A
We have two positions open for either a registered HT or HTL at UAMS. We are in Little Rock Arkansas in the middle of the state and it is a gorgeous area. We have great benefits and a competitive salary range. We are currently reorganizing the Histology Laboratory and seeing increases in our

[Histonet] Re: Fixation time

2012-12-05 Thread Teri Johnson
Hi Daniela, We have successfully stored in 70% ethanol without issue. The only published account I have seen of any issue with alcohol storage is it causes vacuolization in brain. I would not really want to store in fixative any longer because you will continue creating cross links and that

[Histonet] Re: Tissue Processors

2012-12-05 Thread Teri Johnson
Dear Tim, Here's the deal, they all work. When buying new, I bought the VIP5 due to its great reputation. It was and still is a workhorse. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752

[Histonet] job opening in PA

2012-12-05 Thread Hutton, Allison
Doylestown Hospital is Doylestown, PA has the following position open: Job Title:Histology Tech - 1123 Req: 2012-2409 Location: Department: Laboratory Schedule: Mon-Fri varied start times

Re: [Histonet] Re: Tissue Processors

2012-12-05 Thread Rene J Buesa
If I may, I would like to add something: do you remember the sales success of the VW Beetle? Why was it a success? Because of its simplicity. They did not even have a gasoline gage and if you ran out of gasoline there was 1 extra gallon reserve that you opened with a handle underneath the

RE: [Histonet] Re: Tissue Processors

2012-12-05 Thread Ellenburg, Deborah
Well stated - agree 100%. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, December 05, 2012 11:55 AM To: Teri Johnson; histonet@lists.utsouthwestern.edu Subject: Re:

[Histonet] RE: processing protocol using isopropyl alcohol, mineral oil paraffin

2012-12-05 Thread Davis, Cassie
I'd really like to see a copy of the protocol if any one would be willing to share. We are still using reagent alcohol xylene, the waste disposal prices are a significant portion of our budget. Cassandra Davis cda...@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any

RE: [Histonet] RE: processing protocol using isopropyl alcohol, mineral oil paraffin

2012-12-05 Thread Lynette Pavelich
Yes, please share! Thanks! Lynette From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Davis, Cassie [cda...@che-east.org] Sent: Wednesday, December 05, 2012 1:41 PM To:

[Histonet] Leica Bond RX

2012-12-05 Thread Patsy Ruegg
Good Day everyone, Is anyone using the new Leica Bond RX research IHC/ISH autostainer? If you are I would love to hear any feed back on it you may have. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Phone:

[Histonet] Tissue Processors

2012-12-05 Thread Michelle Lamphere
We currently have three Excelsiors and I would have a hard time going back to any other processor. I really appreciate the reagent management of this machine. We have virtually eliminated all hazardous reagent exposure during reagent exchange. I think their customer service is outstanding as

Re: [Histonet] processing protocol using isopropyl alcohol, mineral oil paraffin

2012-12-05 Thread Maria Mejia
I'd like to be included in the protocol processing exchange too! Best Maria Mejia On Dec 5, 2012, at 10:55 AM, Lynette Pavelich wrote: Yes, please share! Thanks! Lynette From: histonet-boun...@lists.utsouthwestern.edu

Re: [Histonet] Trichrome

2012-12-05 Thread Massimo
Hi, In Trichrome staining not excellent results are always obtained by fixing in NBF alone. Often the advised fixatives are: - Sublimate-acetic - Zenker's liquid - Telleyesnicky' s liquid - Müller's liquid added with a formalin solution - or re-fixing  by Bouin's liquid I'm talking about

[Histonet] Re: Histonet Digest, Vol 109, Issue 6

2012-12-05 Thread Madeleine Huey
-Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Tuesday, December 04, 2012 3:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processors Hello Tim, Here are some of