[Histonet] moleculer testing

Happy Monday Histonetter Who out there in Histoland is doing the testing for BRAF, KRAS, ALK, and HER2neu and what testing methods are you using. I prefer not to use FISH. Would rather have a permanent record of the slides. Any information is welcome. Have a great week. Cindy Cindy Pyse,

[Histonet] moleculer testing

Hi Cynthia, We do PCR for KRAS and BRAF. You cant do these by FISH anyway, the mutations are too small. We do FISH for ALK and HER2. For ALK, there is no antibody that can detect all the positive cases. ALK FISH is the only method that can detect ALK break aparts regardless of the fusion

AW: [Histonet] moleculer testing

Hi, this histolab does BRAF, KRAS, EGFR with pyrosequencing and pcr; her2-amplification with DDISH on Ventana-Benchmark and ALK with FISH. Gudrun Lang -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag

[Histonet] Chandler AZ HT Position

Great opportunity for a Histotechnicians' in a brand new laboratory! Tri- City Colo-Rectal Surgery in Chandler, Arizonais looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must meet the following criteria: * Meet CLIA Grossing Requirements :

[Histonet] Follow up question for embedding centers

Hi Everyone: I was wondering if anyone has had experience using two other embedding centers that I have received information on? One is the Medite VALIDA. The second is the HistoPro 150. Also, is a stainless steel cold plate and/or work surface susceptible to rust down the road or not?

[Histonet] Her2 Dual ISH and breast processing

Hello all-- My lab is having some problems with inconstant results with our Her2 Dual ISH on the Ventana Ultra. Right now, we know we have a problem with our large breast tissue being under-fixed. There have been many gripes to the PAs and residents about the thickness of the tissue

Re: [Histonet] Her2 Dual ISH and breast processing

Val: Acknowledging that you have a fixation problem is the fundamental step to solving your staining problems. You state that the slices have now a consistent thickness of between 2-3 mm and that is great BUT what about the fixation time? Thin slices is a first step but until you have the slices

[Histonet] Space requirements for AP lab

Greetings All, Are there any JACHO or CAP space requirements for an AP laboratory? Looking for recommended square footage per tech, equipment, and/or work area. Thanks Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406

[Histonet] Qualification in Immunohistochemistry Exam Preparation

Just wanted to send out thanks to histonet for a tip regarding a new study workbook available for the QIHC certification exam! After studying on my own for many months with little direction, I purchased this guide through the Michigan Society of Histotechnologists

RE: [Histonet] Qualification in Immunohistochemistry Exam Preparation

I agree Dawn, the Michigan Society has put out some great publications over the years. Joelle Weaver MAOM, HTL (ASCP) QIHC From: dshaf...@wellspan.org To: histonet@lists.utsouthwestern.edu Date: Mon, 10 Dec 2012 13:39:56 -0500 Subject: [Histonet] Qualification in Immunohistochemistry

[Histonet] Problems with cryosectioning snap-frozen mouse kidney

Hello Histonet members, I was wondering if you could provide some input on a problem we have encountered recently. We routinely section mouse kidneys for IF. The kidneys are cut in half and snap-frozen in Tissue-Tek cryomold in OCT using isopentane/dry ice bath. Lately we have been having

[Histonet] Re: Cryosectioning mouse kidney problems

Thank you, Belinda and Gayle, for your suggestions. Sounds like I will need to double-check the temperature at which the sectioning was done. The tissue is fresh (we used to fix with PFA and then cryoprotect with sucrose but we have switched to using freshly frozen kidneys (snap frozen in OCT)

[Histonet] workbook for preparation for the QIHC

Hi, I tried the Michigan Society for Histotechnologists, but when I clicked on the workbook, was unable to get anything. How can I get in touch with them? Ellen Yee Diagnostic Pathology Medical Group Sacramento, CA 916-448-5873 e...@dpmginc.com

Re: [Histonet] workbook for preparation for the QIHC

Towards the bottom of the MSH webpage on study guides available through MSH, is a link for the application form. www.mihisto.org click on Education click on Study Guides Peggy Wenk -Original Message- From: Ellen Yee Sent: Monday, December 10, 2012 6:26 PM To: