I have a question regarding scheduled maintenance of rotary microtomes. I run a
simulation lab for students of lab technology. Part of their histology rotation
involves learning how to produce acceptable sections on the microtome. I
currently have 3 Leica rotary (manual) microtomes, which are
Melanin can be distinguished from DAB by counterstaining with Azure B, which
gives a dark green color to the melanin. We've used the procedure below with
success:
Immunoperoxidase technique modified by counterstain with azure B as a
diagnostic aid in evaluating heavily pigmented melanocytic
For your usage level it is unnecessary an annual maintenance. As a matter of
fact I am sure you will be able to use those Leica microtomes for many years ar
the same usage level without needing any capital maintenance.
René J.
From: Allen Keeping D. allen.keep...@albertahealthservices.ca
To:
Hello,Our lab is getting ready to construct TMAs to optimize new Immuno
stainers. We purchased the Arraymold TMA instrument and was wondering if
anyone has used it for this purpose? It looks fairly easy to use.
ThanksJimmy
Dear Histonetters
Hi.
Now I am doing immuno-stain of the cells from bronchioalveolar lavage(BAL).
The primary antibody is for detecting some intracellular
antigen(antigen in cytoplasm).
So I think permeabilization process may be necessary after fixation by 4% PFA.
Does anyone know whether we can
