[Histonet] holly nuclei bat man

2013-01-05 Thread Patsy Ruegg
Happy New Year everyone! Can we start a discussion once again on what causes holes in nuclei? It is pretty clear in my experience it has something to do with heating at least for me with antigen retrieval. When I do HIER at ph 8 especially I am seeing a lot of holes in nuclei. H&E on same ti

Re: [Histonet] Re: Pathologists' Names on H&E Labels

2013-01-05 Thread Daniel Schneider
You can get Sharpies and highlighters in at least 7 different colors. We color code the pathologists (Dr's Red, Blue, Pink, Orange, Purple, Green, and Yellow.) The Sharpies and highlighters are distributed in the appropriate departments, and the slides and paperwork get a quick mark of color to f

[Histonet] Re: CAP guideline for fixation time for ER/PR/HER2

2013-01-05 Thread Bob Richmond
Time of fixation before ER/PR/HER2: I haven't had much luck with getting this information. Nobody wants to deal with the communication issues. Needle biopsy specimens should be popped into formalin as soon as they are out of the patient, and the time recorded. What you can't find out is whether t

[Histonet] Fw: PSCA Primary antibody

2013-01-05 Thread dusko trajkovic
Hallo Histonetters, Happy New Year!! I'm looking for information on a PSCA (Prostate Specific Cell antigen) antibody that will work both in human and primate. If are are using this antibody, please contact me. Any information would be appreciated. thanks Dusko Trajkovic 858-638-6202 ___

[Histonet] Re: Pathologists' Names on H&E Labels

2013-01-05 Thread Bob Richmond
Pathologist's name on the slide? I'd just like to see the patient's name on the slide. It helps me not mix up cases, but the old-timer histotechnologists really resist it. I guess you can legal-beagle reasons not to put the pathologist's name on the slide, but I don't see anything wrong with it. S

[Histonet] Re: Helicobacter stains and controls

2013-01-05 Thread Bob Richmond
There are four broad choices for staining Helicobacter. 1. Rely on H & E alone. Some people claim it works, but I can't find them unless they're all over the place. 2. Thiazine dye methods. The simplest is the blue dye mix Diff-Quik II (or a generic equivalent, all of which work in my experience)