[Histonet] Disposable blade holder

Hi All, I am searching for a disposable blade holder for an older cryostat. It is a Heidelberg HM 500 (Microm). Thanks, Jennifer MacDonald Mt. San Antonio College ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.e

[Histonet] Mouse eye histology

Hello, I need some help on how to remove the sucrose from mouse eye balls also I need a good protocol for fixation and sectioning of mouse eye balls (without detaching the retina from the rest of the eye section) for Immunohistochemical staining. Any help I would really appreciate it Thank you

[Histonet] RE: QC for fire shower

You mean chemical / fire shower? Our hospital has a guy test it once a month and record it. And record on a tag on the shower the date it was tested? Tim Morken UCSF PATHOLOGY -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwest

[Histonet] QC for fire shower

Does anyone have a QC log on the yearly fire shower testing they'd like to share? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Leica 4020 linear stainer

Could I get some feedback on linear stainers?Has anyone had any experience with the Leica ST4020? We are going to purchase a linear stainer for a new MOHS laboratory. Thanks, Sandy Harrison Histology Supervisor VA Health Care Systems, Minneapolis 612-467-2449 _

[Histonet] RE: CAP guideline for fixation time

We have it documented within the APLIS, when the order is placed within our EMR,, for requisitions we have them document them on the requisition at the time it is taking place with a sticker,, but since the most of our orders are electronic it is part of the workflow, this is done for all specim

[Histonet] Histology/Cytology Jobs in New York

Allied Search Partners has the following positions available for permanent placement. Please contact me if you would like more details. Grossing Histotech Monday-Friday 9pm-5:30am or Monday-Friday 7pm-3:30am Westchester area *NY Clinical Lab or Histology License Required Cytoprep Technician Mon

[Histonet] RE: CAP guideline for fixation time

Our OR and Breast Health staff have done pretty well getting on board with this. We rarely have one that hasn't been documented. We have a place on the requisition (located just under the date field so they will see it) where time out of body (for the cold ischemic time), and time into formal

[Histonet] EGFR primary

Hello everyone, Can you please share with me the EGFR primary antibody you use and what pretreatment you use with itthanks in advance!! Colleen Forster ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/ma

[Histonet] TMA Cutting Problems

I just read on the Arraymold website that I am probably using the wrong type of paraffin for constructing my TMAs. I used Richard Allen Type 9 but they recommend Paraplast X-TRA from Leica or Blue Ribbon from Surgipath. I hope a different paraffin solves the issues.

RE: [Histonet] CAP guideline for fixation time

We have a label that we place on the original requisition documenting the time the specimen was placed in formalin and the time it came out of formalin(changed stations on tissue processor). When the transcriptionist transcribe the gross dictation they give the total number of hours the specimen

Re: [Histonet] TMA Cutting Problems

Probably the paraffin wax surrounding the cores has not infiltrated them perfectly and you have ended with a TMA block that cannot hold the cores adequately. My suggestion is to melt the block again and leave the cores in melted paraffin during at least 1 hour and prepare new blocks that will ha

[Histonet] TMA Cutting Problems

We constructed some TMAs for Validations and when I tried to section the TMA blocks the sections shred. It's really hard to get a nice ribbon or even a nice section. I don't know what I am doing wrong. Has anyone had problems cutting TMAs before? Any solutions? We are using the Arraymold i

[Histonet] staining site of bone deposition on live specimens

Earlier I submitted a question to histonet to ask about labels that can be used to bind bone at the site of bone formation in aquatic amphibians. Robert Schoonhoven kindly sent a response stating that tetracycline has been used in mammalian systems. I also recently found a paper (citation

RE: [Histonet] Pathologists' Names on H&E Labels

We have recently moved to printed/barcoded labels for all of our slides. Each pathologist has a mnemonic in our LIS (Meditech) that we use when assigning the case. This mnemonic is picked up and printed on the slide label. It has been a real help, especially on recuts and stains. We no longer