I am trying to purchase thrombin for cell block preparation, and have
been running into dead ends. Can someone give me their vendor
information?
Laurie Best
Histology Supervisor
Sunrise Medical Labs
250 Miller Place
Hicksville, NY 11801
(631)435-1515x1018
This message contains
Hey Julia...
WE do SDH routniely on our muscles every week and they work just fine. Can you
forward your protocol? Id like to see the difference in them if any?
I remain yours truely,
Candice Camille
From: Celebre Julia celeb...@hhsc.ca
To:
I know most embedding machines put out heat due to the block warming
chamber, paraffin vat and compressor of cooling consol. But those of you
who have experience with this machine, do you think it seems hotter than
other or does it have issues with retaining a constant temperature? Any
thoughts..
Does anyone have the phone number and/or email for Robin Simpkins at Biocare?
Thanks,
Laurie Colbert
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Hello All,
We just got a TBS APT1-120 tissue processor donated to us. I am would like
to hear from anyone about the single mode verse the group mode of
operation. What your opinoins about using one verse the other. I hope to
set this up this week and would like advice on which is better.
Hi everyone,
Just have a quick question for all of you out there in
histo-land. Have you ever bought or worked with a gravity convection oven (the
thermo shandon 20GC), for drying of slides?? If so..how was it? Did it properly
dry all of your slides, did it keep temp?? I am
Natalia:
I just cannot understand the concept of gravity convection oven because if
you say gravity by definition that will mean that the heat will work by
gravity and any heated air goes, also by definition, against gravity because
any heat air will go UP and not down, hence the hot air
Dear Laurie,
We use thrombin and plasma to form our cell blocks. We order
the Topical Thrombin 5,000 IU from King Pharmaceuticals.
Hope this helps,
Natalie Nagy (HT)ASCP
Holyoke Hospital
Holyoke, MA.
CONFIDENTIALITY NOTICE: This email communication and any attachments
There is a reason to distinguish the type of oven but the terms are used
loosely and so cause confusion.
Convection is used to describe in nature to describe the natural circulation
of heat and can be of natural gravity convection in which heat rises, or
forced in which some kind of external
Hi Ian,
You have a good question but can you tell us a little more about your specimen
type? Are you processing these specimens in a fluid based medium (ie, Thin
Prep) or making some cytospins? Any types of bodily fluids that are collected
will vary in cellularity and any microbes that could
We have an autopsy on a young child. I have a working diagnosis of possible
Reye's syndrome and need an Oil Red O stain on a section of formalin fixed
liver (not processed) to confirm microvesicular hepatic steatosis. Does anyone
do this stain, and could I send the tissue or unstained slides
I have found that the major problem with most of these enzymehistochemical
stains is that the sections should not be allowed to sit too long at room
temperature before staining. Either stain immediately on sectioning or store at
-70oC.
The enzymes tend to deteriorate quite rapidly at room temp.
Laurie Best, Histology Supervisor at Sunrise Medical Labs, Hicksville NY asks:
I am trying to purchase thrombin for cell block preparation, and have been
running into dead ends. Can someone give me their vendor information?
I know about using thrombin to clot plasma to form cytopathologists'
David,
(From Doug Reye's old Laboratory).
I have found the following technique the best:
Cryotomy of Formalin Fixed Tissues
Sometimes frozen sections are requested on tissue that has been fixed in 10%
formalin. Cutting frozen sections of formalin fixed tissue can be frustrating
at times due
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