[Histonet] HER-2

2013-02-01 Thread Wilson A
      Hi,   Our pathologists are concerned we may be reporting too many 2+ HER2’s. Can someone  help with this?   Thanks,     Wilson ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/hi

[Histonet] Staining for Phospholipids

2013-02-01 Thread Duraine, Lita
Hello Histoland, Someone needs help in our lab with some fluorescent staining. He is staining drosophila retina with antibody PI4.5P2, or PIP2 for looking at Phospholipids around the membranes. He is using 4% Paraformaldehyde and Triton X in his protocol and is getting inconsistent results

Re: [Histonet] Procedure manuals

2013-02-01 Thread Rene J Buesa
Yes, that is the only way you will be able to show everybody knows and will comply with the procedures. This is of paramount importance for the annual evaluations. If you change any procedure, that will be signed; if procedures remain the same there is no need. René J. From: Gail Marcella To:

[Histonet] RE: Procedure manuals

2013-02-01 Thread Ian R Bernard
Pre CAP every two years or as needed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gail Marcella Sent: Friday, February 01, 2013 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Procedu

[Histonet] Georgia Society of Histotechnology

2013-02-01 Thread Zimmerman, Billie
This is a friendly reminder to renew your free membership with the Georgia Society of Histotechnology. If you have any questions or concerns, please send me an email at zbilli...@gmail.com Thanks, Billie Zimmerman MT(ASCP)QIHC Secretary of GSH Augusta State Universi

Re: [Histonet] 4% Paraformaldehyde

2013-02-01 Thread Paula Sicurello
Rhonda, What Tim says is true, it all depends on what the tissue is going to be used for. Provide more detailed information to get a more detailed answer. Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South,

RE: [Histonet] 4% Paraformaldehyde

2013-02-01 Thread Morken, Timothy
Rhonda, Your question is a little to general. 4% formalin made from paraformaldehyde is similar to 10% formaldehyde. So, the question is, for what are you storing the tissue? If it is for morphology studies, then it can be weeks or months. If it is for immunochemistry then days to weeks is gen

Re: [Histonet] 4% Paraformaldehyde

2013-02-01 Thread Garlick, Rhonda
I got one response from my question below. Does anyone else have hard and fast rules with regards to the length of time tissues should/can be stored in 4% Para? Thanks, Rhonda On 1/22/13 1:08 PM, "Garlick, Rhonda" wrote: >I've been doing some on-line searching with regards to the length of

[Histonet] Procedure manuals

2013-02-01 Thread Gail Marcella
Hi - Does anyone know if everyone that works in the lab has to sign off on every procedure in the manual, every single year??? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Benchmarking

2013-02-01 Thread Elizabeth Cameron
Histonet Users, Scientific Services at The Jackson Laboratory is conducting benchmarking surveys in an effort to develop an understanding of the best current practices offered by peer institutions. The goal is to gather quality data that can be used to assist us and all survey participants with

RE: [Histonet] Expiration dates of ammonium sulphide

2013-02-01 Thread Morken, Timothy
Am sulfide is generally used in excess in procedures so the concentration is not critical. My approach is to buy the smallest amount possible and use it up before the expiration. Then there is no need to worry about activity, expirations, extending life, etc. For instance we use it for muscl

Re: [Histonet] Expiration dates of ammonium sulphide

2013-02-01 Thread Rene J Buesa
Ammonium sulfide loses its activity with time. You will have to test it. Please review a recent thread on this subject in HistoNet. René J. From: Tony Henwood (SCHN) To: histonet Sent: Thursday, January 31, 2013 6:39 PM Subject: [Histonet] Expiration dates of ammonium sulphide Hi all, I have

[Histonet] Cardiac tissue freezing

2013-02-01 Thread Laura Avogaro
Hi everyone! I have to flash freeze human atrial tissue biopsies. Does anyone can suggest me a protocol or some useful tips for this procedure in order to preserve the tissue architecture without artifacts formation? If is possible I prefer to avoid the use of any fixative. Thanks in advance Laur