I would love to hear any feedback that you get from this query.
Michelle M Lamphere, HT (ASCP)
Senior Tech, Histology
Children's Medical Center
1935 Medical District Drive
Dallas, TX 75235
Office :214-456-2798
Histology: 214-456-2318
Fax: 214-456-0779
Could I get the opinions of any labs
Hey Kathy,
It is not considered a High Complexity test on the staining side of it. You
don't need any special degree or certification, I know we all want our
profession to fetch more money and respect and a good way is make a mountain
out of molehill to use an old saying.
Now here is
Hello all,
I was wondering what most people use as the first reagent after the formalins
on their tissue processor? We have always used a sequence of 70%, 80%, 95%,
and 100% but is anyone using 80% or even 95% to start their dehydration?
Thanks in advance.
Tom McNemar, HT(ASCP)
Histology
I had to sign up again for the Histonet so I am not sure if this question went
out so will resend. thanks
Hello Histonetters, We are trying to embed a polycarbonate device with soft
tissue attached to look at the implant interface. The problem is that with
several standard protocols for pmma
I only hope that Tim's posting sets this issue to rest. The histotech doing
IHC, FISH, or grossing and some other complex tasks has to have special
training and studies because all those are high complexity tests.
Even those histotechs reading FISH results (counting the reactive nuclei for
If you look on the paint thinner isle at your local Lowe's. You will
find Xylene on the shelf. The first time I saw it was a shock. Wonder
if the home improvement weekend warriors know what they are getting all
over their hands?
Lisa White, HT(ASCP)
Supervisory HT
James H. Quillen
You have to be careful or the salts will precipitate out of the formalin if you
start too high. I wouldn't go any higher than 70.
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta,
I usually started with 80%EthOL but it does not harm starting with 70%EthOL
and it is even better, from the theoretically view point. So if you have the
space in your protocol, keep the 70%EthOL
René J.
From: Tom McNemar tmcne...@lmhealth.org
To: histonet@lists.utsouthwestern.edu
John
Have you tried paraffin? I know that we have been able to section some plastic
devices with paraffin sections, it may be worth a try. Looks like the device
you are working with is compatable with xylene, we have found in some cases
that we need to use a xylene subsititute.
Liz
For sure they do not know, I have asked a few painters. As many histotechs,
they have told me that the like the smell of xylene.
René J.
From: White, Lisa M. lisa.whi...@va.gov
To: histonet@lists.utsouthwestern.edu
Sent: Thursday, February 7, 2013 10:23 AM
Subject: [Histonet] Xylene/Paint
I have to admit, I don't mind the smell:) But I also like the smell of
gasoline, and I know both are bad for me!
Thanks again to everyone who has offered feedback on this topic. Rene, I have
printed out a few of the articles you've written and am anxious to research
these methods more. Thanks
I don't know if the auto parts stores carry xylene, but it is an awesome
degreaser. Learned that from my mentor back in the 70's.
Victor
Victor Tobias HT(ASCP)
Clinical Applications Analyst
Harborview Medical Center
Dept of Pathology Room NJB244
Seattle, WA 98104
vtob...@u.washington.edu
I'm sure my wife would love that.
Victor Tobias HT(ASCP)
Clinical Applications Analyst
Harborview Medical Center
Dept of Pathology Room NJB244
Seattle, WA 98104
vtob...@u.washington.edumailto:vtob...@u.washington.edu
206-744-2735
206-744-8240 Fax
=
If you ever need to degrease any auto part, just place it in water, add liquid
soap to about 5% conc., and heat it until boiling and let them in boiling water
during 5 minutes. Wash with running tap water and dry.
They will degrease even better tan with xylene.
René J.
From: Victor A. Tobias
I agree with Joyce on this: Formalin salt precipitate tends to become more
common if you start above 70%. WE use a 70%, then 80% and two 95% in our
process here.
Very Respectfully,
Jeremiah C. Preszler, MSgt, USAF HT (ASCP)
Flight Chief, Anatomic Pathology
959 CSPS/ SGVLH
WHASC
JBSA-Lackland
And Dawn is the best...
And I am in no way linked to this product!!
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
This e-mail, including any attachments is the
Tom, We start at 80%.
Tim Morken
UCSF Pathology
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Sent: Thursday, February 07, 2013 7:15 AM
To: histonet@lists.utsouthwestern.edu
Subject:
FISH is definitely high complexity. The tech who scores the slide must
meet the qualifications to perform high complexity testing. I understood
Kathy's question to be about the person who loads and runs the
slide stainer.
I still say give CAP or whomever you accrediting agency is a call and see
Hi Tom:
I deal exclusively with post-mortum brain tissue, so my situation may
not apply to you.
I do not use formalin on my processor, since the half brain used for
brain-cutting has already been thoroughly fixed.
So, I have the luxury of using 30%, 50%, 80%, 95%, then three 100%
How do you dispose of old tissue blocks after the 10 year hold period? Can
old glass tissue slides be sent for recycling?
Thanks in advance
Joe Maslanka BS, CT,HT (ASCP)
Anatomical Pathology Technical Supervisor
St Peter's Hospital,MT 59601
(P)(406) 447-2406
(F)(406)444-2126
Give thanks for
Forwarding a message I received:
Hi Roger,
I've been using the Lieca Bonds for about 4 years now and I wouldn't trade for
anything. The lab I am currently at changed everything from Ventana over to
Bonds about a year and a half ago, and we have NO regrets!
Bonds are faster, more flexible with
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Hello all,
I was wondering what most people use as the first reagent after the
formalins on their tissue processor? We have always used a
Has anyone come up with a documented and referenced procedure for
decontamination of a cryostat using 37% Formaldehyde?
I pulled up old notes in the Histonet archives and saw that Tim Morken
was working on this but that was a number of years ago.
Thanks, Kim
Kimberly D.
Hello everyone,
I have a co-worker in the research department that would like to know if anyone
has ever combined and IF stain with chromogenic IHC staining. His theory for
this is that IF shows better nuclear staining from an image analysis stand
point. If you have heard of this can you send
Joe,
We redbag all paraffin blocks for medical waste disposal.
We use large sharps containers for disposal of glass slides.
Very Respectfully,
Jeremiah C. Preszler, MSgt, USAF HT (ASCP)
Flight Chief, Anatomic Pathology
959 CSPS/ SGVLH
WHASC
JBSA-Lackland AFB, TX 78236
(210) 292-5519
Anybody else having problems with PIN 4 staining (Biocare)? We are getting
brown background staining, even on the glass itself where there is no tissue.
After many years of fantastic results, this problem has occurred recently. Our
AMACR is also a lot fainter than it used to be. We've switched
Sent from my Verizon Wireless BlackBerry
-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu
Sender: histonet-boun...@lists.utsouthwestern.edu
To: histonet@lists.utsouthwestern.edu
Reply-To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 111, Issue 8
Send
What are people's thoughts/experience with different fixatives for eyes
(rat, mouse and rabbit mostly).
We currently use modified Davidson's solution, but the pathologist is
considering going to Davidson's.
I guess there has been some difficulty with cutting due to the lens not
getting fixed
How long are you leaving the eyes in the mDavidsons? We have found 24 hours
works extremely well.
-Original Message-
From: Robin Dean robin_d...@compbio.com
To: histonet histonet@lists.utsouthwestern.edu
Sent: Thu, Feb 7, 2013 6:52 pm
Subject: [Histonet] best fixative for eyes
What
Love the bond it does not break down very often for me and service is great i
can run all the antibodies i want for me even research animal because i have a
more open research unit cleaning the covertiles is a little tedious but we make
it work and replace them with new ones often beats
Really 1:4?___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Hello,
I have previously cut frozen sections and post-fixed in methanol for 2
minutes and got OK staining using mono- and polyclonal antibodies to
the lipid galactocerebroside (Galc). This has also been the case with
MBP (myelin basic protein). I am now using PFA fixed tissue and then
washing in
The bond is great. A lot less waste to deal with and the maintenance is s
easy. Less than 10 minutes. No tubing to unclog and disinfect. And the
operating cost is much cheaper.
And to top it off, it won't even give you the option to start a run until
everything is right. No going back and
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