Patti,
The best antibody (much better than any rabbit, goat or even the same 1A4 clone
conjugated with FITC) are:
Mouse monoclonal Anti-alpha-smooth muscle actin Cy3 conjugated, Sigma
cat.No.C6198.
Works in dilution 1:500-1:2000 on formaldehyde fixed cell cultures,
cryosections (both from fresh
I love Sigma DAB Tablets, they come in 1-5 and 15 MLs and you have to dissolve
in water. The only problem is that they do not have any expression date. Soofia
From: Blazek, Linda lbla...@digestivespecialists.com
To: Sandra Cheasty cheas...@svm.vetmed.wisc.edu;
I am asking this for a researcher. Please respond to her at
klar_elizab...@columbusstate.edumailto:klar_elizab...@columbusstate.edu. Her
name is Ely Klar.
She is looking for any form of estrogen antibody that works on bass fish
gonads. Vendor's responses welcome.
Shirley A. Powell,
We would need to see the entire set of steps as there may be a variety of
causes.
Robert Schoonhoven, HT/HTL (ASCP)
From: Nicole Cosenza ncose...@siumed.edu
To: histonet@lists.utsouthwestern.edu
Sent: Thursday, March 7, 2013 12:50 PM
Subject: [Histonet]
Section should not dry in any stapes.
Tahseen
On 2013-03-07 22:50, Nicole Cosenza wrote:
Hello all:
I recently did IHC on paraffin embedded slides via the DAB method.
Upon addition of the DAB, the entire section turned brown. I know
this
is not true staining, as I don't expect a high
Add another Leica to your list
René J,
From: kgrob...@rci.rutgers.edu kgrob...@rci.rutgers.edu
To: histonet histonet@lists.utsouthwestern.edu
Sent: Thursday, March 7, 2013 2:32 PM
Subject: [Histonet] Microtome upgrade, planning stages...
We are starting to look into upgrading to higher-end, but
Thanks to everyone for your suggestions and protocols for the IDH1 antibody.
It helped immensely and now I don't have to beat my head against the wall
anymore!
Billie Zimmerman MT(ASCP)QIHC
Laboratory Clinical Operations Mgr.
BF 117
706-721-5617/3630
Fax: 706-434-4883
Augusta State
Our histology SOPs start with H- (to distinguish them from other area SOPs)
then each is assigned a unique chronological 4 digit ID number followed by the
version number.
For example: H-0001.00 where 0001 is the number for a specific SOP and .00
refers to the original version. The number for
Leica's CoolClamp, or Thermo's Cool Cut? These sound like a possible good
solution for the inevitable days when the aging AC plant here can't keep
up with the summer heat.
Thanks so much!
Kathleen
Principal Lab Technician
Neurotoxicology Labs
Molecular Pathology Facility Core
Dept of
