Hi Histonetters
Does anyone use MUC3(mucin) antibody that is working on FFPE human
tissue?
Many Thanks
Marilyn Tyler
Medical School UCT
Dept of Surgery J50/26 or 30
Surgical research. OMB
Groote Schuur Hospital
Observatory
021-4066227
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UNIVERSITY OF CAPE TOWN
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I would like to have some informations about fetal brain fixation..
In general, it is in use the fomalin, but during the cut it is possible to
see that in the deep layer the formalin doesn't arrive...
So, Could you give some suggestion to improve this technique?!
Thanks in advance
Best
Good morning,
I have a tissue tek embedding center. The hot plate no longer works.
I have been told I can not get it replaced until July and it is too expensive
to repair. I have looked into barrowing or getting a loaner from someone who
has a back-up. That has not worked. Anyone
The fixation is not complete because fixation time is too short.
Please go to http://www.histosearch.com/rene.html where you can find two
articles about formalin fixation where this issue is discussed.
René J.
From: Giulia Zunino giuli.zun...@gmail.com
To: histonet@lists.utsouthwestern.edu
Maybe one of the used equipment vendors would consider a short-term lease with
option to buy.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of MARCELLYN A.
STONE
Sent: Wednesday, March 13, 2013 7:14 AM
Good Morning!
CD68 (for the BOND)is backordered until May and I am looking for
recommendations - Cellmarque or Biocare? Is there something better?
I appreciate your input.
Nancy
Dubuque, IA
NOTICE: This email may contain legally privileged information. The information
is for the use of only
Rene is correct, formalin fixes slowly so more time is needed. Raising
the concentration of formaldehyde will not help. Adding a small amount
of glutaraldehyde (0.25 to 0.5%) will fix more quickly but may have a
negative effect on immunostaining.
Geoff
On 3/13/2013 6:31 AM, Giulia Zunino
We use Ventana's ready to use for the Ultra, but it is clone KP1. You can get
the same clone in a concentrate from Cell Marque.
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message-
From:
Hello,
I've read some papers which reference the combination of smooth muscle cell
actin immunostaining with a Verhoeff's elastin counterstain.
Has anyone ever done this and if so, do you have protocol you'd be willing
to share?
I'm staining mouse and rat lung and have a SMC protocol working
Patti,
The immunostaining probably was not wiped out, rather just covered up with the
red color of the VG counterstain. You might try a chromogen that expresses in
a different color. Or, you might leave off the van gieson part of the EVG
(staining only the elastic tissue) and pick a
Patti,
You're using a mouse monoclonal antibody on mouse tissue, so be aware that
the secondary link antibody will bind to any mouse IgG it finds in the
tissue (normal plasma cells, IgG in normal sera present inside blood
vessels, or as infiltrating interstitial normal sera, etc.), not just to
Hi all,
I have some issues with Shandon Pathcentre. Though the machine is kind of old
it was very perfect in shape and running good.
I am posting this request to see if any of other labs faced this issue before
and if any one can give me suggestions.
It was running very fine and perfect, until
We indeed discussed placenta encapsulation on Histonet about a year ago.
http://lists.utsouthwestern.edu/pipermail/histonet/2012-March/061671.html
There are also placental homeopathic remedies:
http://lists.utsouthwestern.edu/pipermail/histonet/2012-March/061671.html
though I'm not sure that
Good afternoon,
In the past I have been using a 40mM Alizarin Red S Solution from
Millipore to stain my cell cultures but I am now seeking an alternative.
I plan on preparing fresh Alizarin Red S by adding the powder to 100ml
of distilled water. In reviewing the literature, I have come across
On May the 4th, 2013, Polysciences, Inc. is hosting a full day educational
event focusing on the Histological Applications Techniques for Bone,
Biomaterials and Medical Device Implants. On behalf of the speakers (Bob
Skinner, Philip Seifert, Valantou Grover and Jack Ratliff) contributing to
Nancy,
The red of the classic VG counterstain stains the collagen. Muscle will appear
yellow. If the yellow is obscuring the brown DAB then after VG staining wash
the slides in water. This will remove the picric acid staining leaving the
muscle brown.
Since Nova red is red (I assume since I
http://www.petfriendly.es/llahg/hxlke.wcdarfmxxhbo
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We always used a 2% Alizarin red S (never tried it with 1%. I'm guessing
that you would just have to leave it in longer.)
We pH it to 4.1-4.3 with 0.5% ammonium hydroxide. I don't remember what the
pH is when we begin, but note, we're adding a BASE to bring the pH up to
~4.2. So alizarin red
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