RE: [Histonet] Possible continuing automatic staining problem

2013-03-16 Thread Bartlett, Jeanine (CDC/OID/NCEZID)
Are you talking bout H&E's? If so what hematoxylin and eosin are you using? What protocol? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lisa Sent: Saturday, March 16, 2013 1:46 PM To: histonet@lists.uts

[Histonet] Possible continuing automatic staining problem

2013-03-16 Thread Lisa
Hi We have this continuing problem, don't know if its a staining or possible processing problem, all our slides keep coming out dark blue & muted, we have adjusted the times every possible way, tried cutting thinner, we change our solutions regularly on our VIP processing machine. Our automatic

[Histonet] Re: Histonet Digest, Vol 112, Issue 17 ihc validation

2013-03-16 Thread M. Kap
Check out www.cqpath.com, nice references from a2z ;-) Verstuurd vanaf mijn iPad Op 16 mrt. 2013 om 18:05 heeft "histonet-requ...@lists.utsouthwestern.edu" het volgende geschreven: > Send Histonet mailing list submissions to >histonet@lists.utsouthwestern.edu > > To subscribe or unsubscr

Re: [Histonet] RE: IHC validation

2013-03-16 Thread Kim Donadio
I agree about using known controls however sending cases out for comparison  could be a little costly, it is ideal though. One thing i have done is to go back in the records and pull up old cases that have been sent out for IHC and use those to compare not only the test as a case corrolation but

[Histonet] Best Carmine stain

2013-03-16 Thread Kiranjit Grewal
Hello, Any tips on doing Best Carmine on Frozen sections without water. Can we use mucicarmine solution to pick up glycogen in heart muscle? Thank u, Kiran Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utso

RE: [Histonet] IHC validation

2013-03-16 Thread Troutman, Kenneth A
Hi Laurie, Ideally, you would like to validate both with tissues processed in your laboratory (controls and patient tissue). Purchased TMAs are okay, but they should not be the only thing used. For the instrument validation, I would definitely use tissue that has been processed in your lab an

AW: [Histonet] PCR detection for Mycobacteria

2013-03-16 Thread Gudrun Lang
I'm also interested in these assays. BioProducts in Austria sells a kit vor LightCycler PCR and they state it's good for FFPE. But on their website one cannot see the details. It's for 96 tests. I've found a 24-kit from Qiagen, which should work on FFPE. But the assay is not validated for it. Reg

[Histonet] FW: No Subject

2013-03-16 Thread claudia melidona
http://www.christiankeltermann.de/kqbc/kzcgvmemedmii.roplf ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet