*Hello Histonetters,*
*
*
*I am looking for the staining protocol for thionin/Acid fucshin staining.*
*
*
*I have Rat brain slides which are paraffin embedded and sectioned at 5-7
um. *
*I have had a problem to make a Acid fucshin solution as it does not stick
to the brain slices.*
*Please give
We were given a Hacker Mesei 3655 Coverslipper as donation for our research.
Unfortunately it falters during operation. We badly need a Service Manual for
it to go up and running again. Could you possibly provide us even a PDF copy
of service manual for this item?Kindly help us by offering a
I always thought you could bill for three due to three distinguishable
staining patterns. The two cytokeratins can not be distinguished from
each other, therefore three charges instead of four (PIN4).
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
What do you mean by muted; can you give a little more description? And what
type of tissue, and is this HE?
Sheryl Stephenson | Histology Technician
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On
Well we use a cocktail, so the antibodies are mixed together and so applied at
the same time. All in the same dispenser not two diff one.
Vanessa
-Original Message-
From: Bitting, Angela K. [mailto:akbitt...@geisinger.edu]
Sent: Monday, March 18, 2013 7:49 AM
To: Vanessa Perez;
I am currently completing my capstone project through the University of
Charleston and would greatly appreciate your help in taking a brief 5 minute
survey. This survey is part of my action research paper and relates to various
Lean methods which you may have applied in your histology
Please no recruiter calls
Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State
Gastroenterology Associates is a multi-physician practice located in Northern
Kentucky. Its mission is To provide compassionate, high quality,
cost-effective care to patients' with
Hi everyone, Several people have contacted me regarding the article I mentioned
in my post yesterday. Here are the details:
Immunofluorescence With Dual Microwave Retrieval of Paraffin-Embedded Sections
in the Assessment of Human Renal Biopsy Specimens
AJCP 2013 139:71-78;
Hi everyone,
Would anyone be willing to share information about what clone/company/dilution
you use for p57? We are looking to use this antibody on formalin fixed/paraffin
embedded human placenta.
Thanks,
Jean Taylor, HT(ASCP)QIHC
Immuno Tech
Meriter Health Services
Madison, WI
Jean,
we use Leica's clone Kip2 on placentas on our Bonds; 1:150
Ronnie
Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com
700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
Our histology laboratory is currently having problems with their
specials stains; in particular the silver stains. Can you please tell me
what instrumentation others in the field may be using for special
stains?
Thank you
Debra A Ortiz, BS, MT, HT (ASCP)
Technical Director, Anatomic Pathology
We use the Dako Artisan. What silver stains are you wanting to automate?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
debra.or...@uchospitals.edu
Sent: Tuesday, March 19, 2013 1:22 PM
To:
Hi Erin
We do direct immunofluorescence staining on FFPE kidney biopsies but instead of
microwave antigen retrieval, we use proteinase K (20mg/ml Qiagen Cat # 19131)
diluted 1/10 in Tris buffered saline for 20 minutes at Room Temp. We also
incubate with the antibody (1/5 dilution) for 90
There will be a general membership meeting while we are at Jekyll Island. We
are asking all of you to help us plan the 2014 meeting.
Next year's theme will be HISTOPALOOZA. A palooza is a big, promotional event
that's fun and over the top. We plan to celebrate histotechnology in a big
way.
We are working on validating HER 2 testing in our laboratory. I see that the
guidelines state that we should use 25 - 100 cases to complete our validation.
In the past we sent our Her2 IHC testing to Clarient and they performed the
technical testing and then our pathologists would do the
What did you do to evaluate your system and algorithms. Also the cases of ER
and PR. Negative vs Postive concordance.
Sent from my iPad
On Mar 19, 2013, at 4:57 PM, Vickroy, Jim vickroy@mhsil.com wrote:
We are working on validating HER 2 testing in our laboratory. I see that the
Hi,
We are currently looking to switch out our linear MKII stainer for either a
Leica XL autostainer or the Sakura Tissue-Tek Prisma. Any recommendations?
Are quantity of HEs is increasing and we need adequate equipment to meet
our workload. The incorporated oven seems excellent on both stainers.
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