[Histonet] Pathology requisitions

2013-03-26 Thread Duddey, Aimee
Our facility, as many do now, uses electronic physician order entry. We struggle with the pathology and cytology portion of this. We still require the manual requisition to be able to obtain the necessary information like clinical history, specific specimen info, etc. Does anyone have a good

[Histonet] What's your favorite mouse-on mouse detection kit

2013-03-26 Thread Connolly, Brett M
Hi All, For those of you working in research on animals tissue I'm looking for a consensus of opinions on kits for HRP mouse- on -mouse detection. Would like to avoid biotinylation procedures (i.e.ARK). Thanks, Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck Co.,

[Histonet] RE: What's your favorite mouse-on mouse detection kit

2013-03-26 Thread Manfre, Philip
Hey Brett, We use Vector PK-2200. Usually works well. Phil. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_man...@merck.com -Original Message- From:

[Histonet] HT Position

2013-03-26 Thread Hale, Meredith
Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State Gastroenterology Associates is a multi-physician practice located in Northern Kentucky. Its mission is To provide compassionate, high quality, cost-effective care to pateints with gastrointestinal problems Looking for

[Histonet] Batch Controlling for IHC

2013-03-26 Thread Tim Higgins
Hello All, Have you ever had a Pathologist say, if you have one tissue control (placed on the same slide as the patient tissue) per antibody, per patient case, but there are multiple blocks (same tissue type) from the same patient case, that would be batch controlling? Hope that made

[Histonet] Acid Fast Controls

2013-03-26 Thread Renz, Jason
We are in need of Acid Fast control blocks. Would anybody be interested in trading? We have very good Hpylori and Amyloid blocks. Thank you. Jason Renz HT(ASCP)/lab safety officer St. Alexius Medical Center Bismarck ND, 58506 (701)530-6733 This email may include

[Histonet] WT1 overstaining on Benchmark Ultra

2013-03-26 Thread Dessoye, Michael J
Hello Histonet, I have two strange occurrences that I'm trying to get to the bottom of. Running WT1 from Cell Marque on a Benchmark Ultra using iView detection and the recommended protocol. Two specimens (both of which happen to be pleural fluid) severely overstained, even leaving the slide

[Histonet] Control Tissue

2013-03-26 Thread Laurie Colbert
Does anyone have a good source for obtaining fresh tissue (formalin fixed) for IHC validation? I would need normal and tumor of various types. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab

[Histonet] RE: WT1 overstaining on Benchmark Ultra

2013-03-26 Thread Clare Thornton
I've seen the exact same thing happen...WT-1 antibody from Cell Marque, on Ventana instrumentation. Except we use Ultraview detection. I had 3 WT-1 slides running at the same time. Two were overstained like you describe, one was fine. The repeats were fine. The overstained slides almost

RE: [Histonet] Batch Controlling for IHC

2013-03-26 Thread Bitting, Angela K.
Technically, he would be right. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Higgins Sent: Tuesday, March 26, 2013 10:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Batch

[Histonet] RE: WT1 overstaining on Benchmark Ultra

2013-03-26 Thread HERRINGTON, SHEILA
I have been experiencing the same random over staining issues with WT1 as well. I use the Benchmark Ultras with Ultraview. It started around the same time as a new lot number changed a few months back. I have tried everything, but being so random it is hard to find a solution. Sheila

[Histonet] eye pathology

2013-03-26 Thread Cheryl
Hi Fellow 'Netters-   Happy Passover, Easter, Solstice (...first day of Spring (last week, then it snowed!!)   Is there a reference lab or facility that takes reference cases for eye pathology, specifically corneas?  Do they bill Medicare?   Same question regarding brain (autopsy) pathology?  

[Histonet] ASP 300S Stirrer

2013-03-26 Thread Tucker, Meghan
Hello Histonet, We have an ASP 300S and have found that the stirrer at the bottom of the retort is not staying in place. It pops off of the base during the initial formalin fill and is not providing agitation throughout the process. Has anyone else with this processor had this issue? Any

[Histonet] RE: Pathology requisitions

2013-03-26 Thread Weems, Joyce K.
We have an electronic requisition that looks like the paper that we use in some units. The ORs didn't use it because they didn't have good access to computers from each room. But the GI labs and Breast Health used it successfully. A Pathology is ordered and fields pop up that the info is typed

[Histonet] RE: Pathology requisitions

2013-03-26 Thread Nails, Felton
What system are you using to place these orders in the OR? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, March 26, 2013 2:27 PM To: 'Duddey, Aimee';

[Histonet] RE: Histonet Digest, Vol 112, Issue 28

2013-03-26 Thread Hugh Luk
Hi Sophia, Your message is many hours old, and hopefully you figured out something already. Maybe this will help next time. What stains were you going to do with these slides? Our list of Stain removers may not be compatible with your desired stain. 1) 1% Acid (HCL), ~20 minutes, depending