[Histonet] RE: Histonet Digest, Vol 112, Issue 33

2013-04-01 Thread Parker, Helayne
Hi Michael, I have always wrapped the cell blocks in lens paper. If it is a messy one I wrap it 2 or 3 times in lens paper. Helayne, Hello Histonet, What is everyone doing regarding cell block preparation? We have been using sponges for processing but we've had a recent contamination

[Histonet] (no subject)

2013-04-01 Thread Kharzai, Sadeq
Please remove me from the list. Thanks, Sadeq Kharzai ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] IHC pipettes:

2013-04-01 Thread Jb
What is the best pipette to buy for IHC? What ul range is best used? Thank you- Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] IHC pipettes:

2013-04-01 Thread Elizabeth Chlipala
Most will work fine we have used Biohit and Eppendorf, you just need to take care of them, you need to purchase a stand, most vendors will run some deal when you purchase an entire set. I would purchase 3 - 4 different pipettors. We calibrate every 3 months. - 0.5 ul to 10 ul - 2 ul to 20

RE: [Histonet] IHC pipettes:

2013-04-01 Thread Bea DeBrosse-Serra
Eppendorf and the 4 different sizes, that Liz mentioned. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] slide printer

2013-04-01 Thread Jennifer MacDonald
Surgipath used to have a small slide printer. It would print individual slides. Does anyone know if there is still such a printer available? Thanks, Jennifer Mt. San Antonio College ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] IHC pipettes:

2013-04-01 Thread Rene J Buesa
Eppendorf René J. From: Jb craiga...@gmail.com To: histonet histonet@lists.utsouthwestern.edu Sent: Monday, April 1, 2013 6:36 PM Subject: [Histonet] IHC pipettes: What is the best pipette to buy for IHC?  What ul range is best used? Thank you- Sent from my iPhone

Re: [Histonet] minus 80 freezer safe time in power outage

2013-04-01 Thread Maria Mejia
Hello Tim, When I worked at UCSF, we had five -80C sub zero freezers stored in a cool room AND on those occasions, when a freezer did malfunction, our safe temperature was -50C - mostly because ALL our frozen specimens were brain. We had a protocol in place for checking each freezer (weekly)