Happy,happy Birthday Jules! I started my Histo career working with Jules @
Stony Brook and he was an inspiration to me! I was very lucky to have learned a
lot about this profession from him. Wishing you a great day!!
Michele Margiotta-Watz
Histology Supervisor
BMHMC
-Original Message-
Fellow Histonetters,
I am looking for advice concerning CAP standard GEN 61300 Climate Control.
To those that take humidity records in your lab, what exact % range do you use
for a normal range?
And what do you do if and when you are out of this range?
Thank you for sharing your workflow info
PBM Labs, LLP is seeking a Histology Technician or Histology Technologist to
work nights. The shift can either be 1:00am - 9:30am or 2:00am - 10:30am,
depending on the candidate's preference.
The ideal candidate will possess a high school diploma or equivalent, with a
demonstrated ability to
Hi All
I need help who are working with CD 24 CLONE 528807 Catalog Number MAB
5248 (RD Systems) and CD 44s Pan Clone 2cs Catalog Number BBA10 on
formalin fixed paraffin embedded sections.
1 Reconstitute at .5ml OR 1 ml with PBS OR DW?
2 What is the dilution factor?
3
Lowe's or Home Depot. They don't say science on the side but who cares?
Geoff
On 4/30/2013 10:38 AM, Shirley A. Powell wrote:
You can also check with restaurant supply houses, we have purchased great large
containers with lids for this purpose. Much cheaper than the lab supply houses.
I am very interested in this as well. We have an ongoing issue with
humidity/static. I have been unable to find anything that lists a recommended
humidity level for histology. New construction building codes for labs list
30% - 80% and the equipment manufacturers list 30% - 80% for their
We are also going through humidity issues post inspection. Our values are
based on the specifications in the equipment manuals and are 30%-80%. We
use a digital reader. We have been dipping below that and are working to
get the humidity up. We have brought in a small humidifier but it is not
Hi All,
Looking for antibodies for the A2A receptor for FFPE tissues - any suggestions
would be appreciated.
Thanks,
Brett
Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.commailto:brett_conno...@merck.com
To address Gen 61300, you have to check your instrument manuals, and MSDS for
all your reagents. Create the acceptable ranges and then monitor. Make sure you
create a process to address out of range issues. We use Tutela Medical devices
to monitor both temp and humidity. Our facilites installed
Does anyone have a source for purchasing adenovirus control slides?
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 main lab
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Histonet mailing list
Happy Birthday to Jules. I took my first in-depth immunohistochemistry course
from him - a 2 day seminar. Since then I've read his articles and books
extensively and he has been a great help. A true driver in the field.
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Hi Colleen,
I have used Zinc formalin in the past, and here are some issues we faced.
You must not keep the tissues in Zinc formalin for an extended period of time
or they get brittle.
You must rinse the zinc formalin fixed tissues with water prior to putting them
into any phosphate buffered
Dear Histonetters,
I am looking for hearing from our experienced histonetters regarding the
possibility of staining of immature collagen fibres in animal models of lung
fibrosis. I didn't find any histochemical method in the literature to stain the
immature collagen. I would like to know
Abi
You can perform a Picro Sirius red and that (based upon the literature) will
distinguish between Collagen I/III. You can IHC stain for collagen I and
collagen III in mouse, or possibly look at fibroblasts or collagen precursors.
We have not done this in lung but we have stained for a
Me too Tim when was that had to have been late 80'$ or so. Jules was worried
about med techs taking over ihc from us so he came to the NSH bod and asked us
to develop a certification in ihc for ht's since NSH is not a credentialing
body Freida CArson suggested we petition ascp and that is how
I am out of the office until 05/07/2013.
I will be out of the office on May 6th. If you have a question about an
order or need assistance placing an order, or if the matter is urgent,
please call 877-881-1192 between 8am and 8pm and a VWR Healthcare Service
associate will assist you. For quote
Per Diem histotech needed in Temecula/Murrieta area to cover for vacation,
sick leave, and other arising situations. Must have certification, or be
certification eligible. Position on on-call basis, with possible increase
in hours. Scheduling is flexible, but is current to 4am-8:30am or 12pm-4pm.
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