I rinse off my microdissection tools right after use, so any gross
contamination is gone before it has a chance to dry and stick like superglue. I
then soak them in demi water with some handsoap for 15-30 minutes, and scrub
with an old toothbrush. To avoid damage to the cutting edges, lay the
Hello all,
The technician who does preventive maintenance on our LX-120 tissue
processor recommended that we use xylene as the solvent in our clean cycle.
We are considering using a xylene substitue in the clean cycle and would
appreciate any recommendations.
Thanks very much,
Carla Conway
Posting on behalf of the Suffolk County Chief ME:
The Suffolk County Office of the Medical
Examiner, located in Hauppauge, New York, is seeking to hire a Histology
Technician. Qualified individuals will prepare stains, mount slides, and
perform general histology duties. The candidate will work
Use a 5% solution of a strong lab detergent in isopropyl alcohol. Since the
cleaning cycle is completed at 50ºC that will be enough and you will not have
to expose yourself to xylene vapors.
René J.
From: Conway, Carla cmcon...@usgs.gov
To: histonet@lists.utsouthwestern.edu
Sent: Tuesday, May
Carla
I think most tissue processor vendors recommend xylene for cleaning and do not
recommend a xylene substitute. This may have changed recently but I would
suspect it would void your warranty if you were still under one if the vendor
recommends xylene and you are using a xylene substitute
Carla,
We use Formula 83 in the clean cycle. We do change it more often than you
would xylene though.
Linda
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla
Sent: Tuesday, May 21, 2013
We use Americlear. Have not used Xylene for many, many years.
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
I am curious as to the frozen tissue retention policies in other institutions,
especially in children's facilities. How long are samples considered
diagnostically viable and relevant to patient care? What is done with the
tissue when the time limit is reached?
Our facility is interested in
Michele,
BD Pharmingen, cat.# 556003, mouse monoclonal. I have used extensively the same
clone B56 labeled with AlexaFluor488 and 555 - all they work very well on mouse
and human tissues.
Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
Hi Everyone! The National Society for Histotechnology is back in Las Vegas, NV
for its 6th Annual Summer Symposium June 17-18, 2013! General sessions and
workshops featuring expert speakers will provide you with the tools, advice and
guidance you seek in your professional career. The Summer
Hello to Everyone,
Has anyone had experience doing CK5 and p63 in the rat? Could you please tell
me where you get your antibody? I currently use Biocare's for human and will
try it this week on the rat. I just was wondering if anyone had any other ideas.
Thank you for your help.
Pat
Pat Bell
I have a question which was asked before but I don't remember the answer, how
many CEU's are required by CAP for a histologist in New York City. Thanks in
advance
Madeline Rotger Milanese H.T. BSHCS
500 New Hempstead Rd.
New City N.Y. 10965
845-362-3200 Ext 129
madelin...@yahoo.com
CAP does not put any number as to how much CE is required for working techs.
Just that there is a CE program (54200), and that there is a record of CE in
the personnel records (54400, #6).
GEN.54200 Continuing Education Phase I
There is a functional continuing clinical laboratory education
I use a excelsior and was told to always use Xylene in the cleaning cycle.
Since sub is not as pure as xylene ( obviously) you could be at risk of
developing paraffin clogs that the sub may not get. Using one xylene in the
cleaning has not effected the odor at all in the lab. Good Luck.
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Hi,
We're having trouble staining murine articular cartilage with Safranin O. We
first started using this stain last year and it was working nicely, with strong
staining of both the articular cartilage and growth plate. After a while, the
stain stopped working, with staining only visible in
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