[Histonet] RE: Cleaning Dissection Tools

2013-05-21 Thread Jonathan Cremer
I rinse off my microdissection tools right after use, so any gross contamination is gone before it has a chance to dry and stick like superglue. I then soak them in demi water with some handsoap for 15-30 minutes, and scrub with an old toothbrush. To avoid damage to the cutting edges, lay the

[Histonet] Xylene substitutes for tissue processor clean cycle

2013-05-21 Thread Conway, Carla
Hello all, The technician who does preventive maintenance on our LX-120 tissue processor recommended that we use xylene as the solvent in our clean cycle. We are considering using a xylene substitue in the clean cycle and would appreciate any recommendations. Thanks very much, Carla Conway

[Histonet] Job Oppurtunity: Suffolk County ME Office

2013-05-21 Thread NYSHisto
Posting on behalf of the Suffolk County Chief ME: The Suffolk County Office of the Medical Examiner, located in Hauppauge, New York, is seeking to hire a Histology Technician. Qualified individuals will prepare stains, mount slides, and perform general histology duties. The candidate will work

Re: [Histonet] Xylene substitutes for tissue processor clean cycle

2013-05-21 Thread Rene J Buesa
Use a 5% solution of a strong lab detergent in isopropyl alcohol. Since the cleaning cycle is completed at 50ºC that will be enough and you will not have to expose yourself to xylene vapors. René J. From: Conway, Carla cmcon...@usgs.gov To: histonet@lists.utsouthwestern.edu Sent: Tuesday, May

RE: [Histonet] Xylene substitutes for tissue processor clean cycle

2013-05-21 Thread Elizabeth Chlipala
Carla I think most tissue processor vendors recommend xylene for cleaning and do not recommend a xylene substitute. This may have changed recently but I would suspect it would void your warranty if you were still under one if the vendor recommends xylene and you are using a xylene substitute

RE: [Histonet] Xylene substitutes for tissue processor clean cycle

2013-05-21 Thread Blazek, Linda
Carla, We use Formula 83 in the clean cycle. We do change it more often than you would xylene though. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla Sent: Tuesday, May 21, 2013

RE: [Histonet] Xylene substitutes for tissue processor clean cycle

2013-05-21 Thread Tom McNemar
We use Americlear. Have not used Xylene for many, many years. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] Frozen Tissue Retention

2013-05-21 Thread Bradley, Joshua, D
I am curious as to the frozen tissue retention policies in other institutions, especially in children's facilities. How long are samples considered diagnostically viable and relevant to patient care? What is done with the tissue when the time limit is reached? Our facility is interested in

[Histonet] RE: replacement for Dako's anti-mouse Ki67

2013-05-21 Thread Anatoli Gleiberman
Michele, BD Pharmingen, cat.# 556003, mouse monoclonal. I have used extensively the same clone B56 labeled with AlexaFluor488 and 555 - all they work very well on mouse and human tissues. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203

[Histonet] NSH Summer Symposium

2013-05-21 Thread Jessica Smith
Hi Everyone! The National Society for Histotechnology is back in Las Vegas, NV for its 6th Annual Summer Symposium June 17-18, 2013! General sessions and workshops featuring expert speakers will provide you with the tools, advice and guidance you seek in your professional career. The Summer

[Histonet] CK5 and p63

2013-05-21 Thread Bell, Pat
Hello to Everyone, Has anyone had experience doing CK5 and p63 in the rat? Could you please tell me where you get your antibody? I currently use Biocare's for human and will try it this week on the rat. I just was wondering if anyone had any other ideas. Thank you for your help. Pat Pat Bell

[Histonet] CAP question

2013-05-21 Thread Madeline Gi
I have a question which was asked before but I don't remember the answer, how many CEU's are required by CAP for a histologist in New York City. Thanks in  advance  Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelin...@yahoo.com

Re: [Histonet] CAP question

2013-05-21 Thread Lee Peggy Wenk
CAP does not put any number as to how much CE is required for working techs. Just that there is a CE program (54200), and that there is a record of CE in the personnel records (54400, #6). GEN.54200 Continuing Education Phase I There is a functional continuing clinical laboratory education

RE: [Histonet] Xylene substitutes for tissue processor clean cycle

2013-05-21 Thread Vanessa Avalos
I use a excelsior and was told to always use Xylene in the cleaning cycle. Since sub is not as pure as xylene ( obviously) you could be at risk of developing paraffin clogs that the sub may not get. Using one xylene in the cleaning has not effected the odor at all in the lab. Good Luck.

[Histonet] Unsubscribe

2013-05-21 Thread Thomas Jasper
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Re: [Histonet] Unsubscribe

2013-05-21 Thread Lee Peggy Wenk
For everyone needing to unsubscribe over the summer months while on vacation, or just because . . . keep this email handy. Go to the bottom of any email. The last line is an internet address - starts with http://has the word mailman in it. Click on the link. Scroll to the bottom of the

[Histonet] Safranin O cartilage staining

2013-05-21 Thread Brett Tonkin
Hi, We're having trouble staining murine articular cartilage with Safranin O. We first started using this stain last year and it was working nicely, with strong staining of both the articular cartilage and growth plate. After a while, the stain stopped working, with staining only visible in