RE: [Histonet] antibodies online

2013-05-28 Thread Elizabeth Chlipala
Emily We use biocompare, google, pub med and human protein atlas. I always try to look at some papers if I can, with papers you can review materials and methods and also review images, etc. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592

AW: [Histonet] Movat Pentachrome - Need to enhance Alcian Blue

2013-05-28 Thread Gudrun Lang
Hi Krista, Maybe the bad stainability is more due to oxidation by air of the old block-surface than due to fixation. A deeper slide for comparison would show. I don't know what the alkaline alcohol is good for ? Gudrun -Ursprüngliche Nachricht- Von:

RE: [Histonet] antibodies online

2013-05-28 Thread Connolly, Brett M
I find that the individual antibody search engines I use each provide info from select companies so I generally look at several ...in addition to Biocompare, Google, Human Protein Atlas and PubMed searches. http://www.antibodyresource.com/home http://www.antibodydirectory.com/index2.php

[Histonet] another Movats question (paraffin)

2013-05-28 Thread Molinari, Betsy
Hi, The Alcian Blue in my Movats is very faint or seems to completely disappear. I have been running Movats for years and this is a new problem. I have checked lot #’s everything is the same. The solution is 1% pH 2.7. The slides are in solution for 20 mins, rinse for 5 mins in tap H2O, then

Re: [Histonet] another Movats question (paraffin)

2013-05-28 Thread koellingr
Betsy, Don't know if applicable to this situation but in the past I have on occasion in other labs noted some stain going off and after checking everything, looked at the tap water. In fact I backtracked through the water utility once to see where the water came from, was delivered and what

[Histonet] Leica microtome, buying used vs. new?

2013-05-28 Thread Kate Davoli
Hi All, I need to acquire a Leica RM2235 microtome. I've got a quote from Leica directly, and several quotes from refurbished / used ones too with warranties for 6 months parts and labor. So here's my question: *does anyone recommend strongly against getting refurbished models?* The list seems

[Histonet] Reagent containers for VIP5

2013-05-28 Thread Diana McCaig
Does anyone have spare reagent containers for a VIP5 they are willing to sell. I am looking for 3 to use exclusively for warm water wash. We are located in Ontario, Canada Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] RE: another Movats question (paraffin)

2013-05-28 Thread Molinari, Betsy
Donella, I use Verhoffs. - Betsy Molinari Senior Histology Research Technician 832-355-6524 | bmolin...@texasheart.org | www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 Confidentiality Notice: This message may be confidential and/or

[Histonet] Checklist

2013-05-28 Thread Luis Teran
Does anyone have a checklist for Thermo Shannon Cryotome? Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Temperature strips:

2013-05-28 Thread Jb
Does anyone know if we have to keep our temperature strips from our decloaking chamber/pressure cooker for CAP? We have currently been stapling them to our log. Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: [Histonet] Temperature strips:

2013-05-28 Thread Burton, Lynn
That's what we do. Lynn Burton Animal Disease Lab Galesburg, Il -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Tuesday, May 28, 2013 1:38 PM To: histonet@lists.utsouthwestern.edu Subject:

[Histonet] looking for an IHC tech

2013-05-28 Thread Curt
We're a southern CA lab looking for someone to help grow our IHC dept. as well as contribute with some routine bench work. I'm not going to go into great detail here, please contact if you're interested in exploring the opportunity. PLEASE NO HEAD HUNTERS OR JOB PLACEMENT AGENTS. Curt

Re: AW: [Histonet] Movat Pentachrome - Need to enhance Alcian Blue

2013-05-28 Thread Krista Sider
Hi Gudrun How deep would you consider the oxidation to go? I have tried samples from around 200um into the block. The Alkaline Alcohol converts the alcian blue to monastral fast blue, which is insoluble - this is what i have been told. So locks it in I guess. Doesn't really matter as I don't get

[Histonet] Bone Marrow Issues

2013-05-28 Thread Jordan Phillips
HI everyone. We are currently having problems with all of our bone marrows, mainly clots, washing off the slides. We fix our bone marrows in Zenkers. We use positive charged slides, put the slides in the oven for a minimum of 2 hours and hand depar with no agitation. When we get to the