AW: [Histonet] Processing thin slices of mouse cerebellum

2013-05-30 Thread Gudrun Lang
Is the tissue fixed well enough? At least two days? Processing through graded ethanols with little difference should minimize shrinkage. Instead of 70-96-100, take 50-60-70-80-96-100. Times shouldn't be too short. Fatty brain needs more time for reagens-exchange than other small biopsies.

RE: [Histonet] Extra Money

2013-05-30 Thread Sarah Dysart
I already PRN at a local hospital, but that is usually only one or two shifts a month...I need something to add a third job to bring in extra dollars. I hope you weren't trying to act like I was being lazy or complaining Lee...as I am not either one...I have a new baby and the strain of

Re: [Histonet] Extra Money

2013-05-30 Thread Rene J Buesa
Yes, but only if you incorporate that activity to your c.v. René J. From: Sarah Dysart sdys...@mirnarx.com To: Rene J Buesa rjbu...@yahoo.com; joelle weaver joellewea...@hotmail.com; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wednesday, May 29, 2013 5:05 PM

Re: [Histonet] Extra Money

2013-05-30 Thread Rene J Buesa
Another solution is to do the following: 1- honestly analyze your job performance and qualifications 2- objectively analyze how much your supervisor depends on your work and if with some frequency you take care of business while other colleagues do not. 3- IF both analysis come favorable to you,

[Histonet] antigen retrieval question

2013-05-30 Thread Burton, Lynn
If anyone out there is using a decloaker for antigen retrieval, when doing ihc staining, would you please contact me on my e-mail address? I have been struggling with a BVD test for months. It is not working consistently and I want to explore using this method along with our Benchmark XT

Re: [Histonet] antigen retrieval question

2013-05-30 Thread Rob Day
Is this with an older tissue sample? We don't do antigen retrieval, but we do have a proprietary technique for tissue restoration which can help with problematic IHC studies, especially in older FFPE tissue samples. See here for some examples:

[Histonet] amyloid stain

2013-05-30 Thread Gale Limron
Hello, It's been awhile since we ran an amyloid stain.Today, using Richard Allan Chromaview kit, our staining tech noticed a lot of precipitant in the working Congo Red solution. Any thoughts or advice? Thanks, Gale Gale Limron, HT, CT (ASCP) Union Hospital Dover, Oh This

RE: [Histonet] Extra Money

2013-05-30 Thread joelle weaver
I wasn't talking about appreciation - I meant more like work for hire. It is not much, but can be squeezed in around other commitments. So true, not likely to do much dramatic with your C.V. ! Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Wed, 29 May 2013 13:36:41 -0700 From:

Re: [Histonet] amyloid stain

2013-05-30 Thread Rene J Buesa
Check/change the solution René J. From: Gale Limron ga...@unionhospital.org To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Thursday, May 30, 2013 11:26 AM Subject: [Histonet] amyloid stain Hello, It's been awhile since we ran an amyloid stain.Today,

RE: [Histonet] Extra Money

2013-05-30 Thread joelle weaver
Maybe to the nice folks at NSH :) Joelle Weaver MAOM, HTL (ASCP) QIHC From: sdys...@mirnarx.com To: rjbu...@yahoo.com; joellewea...@hotmail.com; histonet@lists.utsouthwestern.edu Date: Wed, 29 May 2013 21:05:02 + Subject: RE: [Histonet] Extra Money CC: I plan on doing a

RE: [Histonet] Extra Money

2013-05-30 Thread Morken, Timothy
Actually it could be like earning money because I believe a poster presenter gets a free trip to the NSH meeting. Workshop presenters certainly do. Why do you think there are so many repeat presenters!?!? Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of

RE: [Histonet] Extra Money

2013-05-30 Thread Morken, Timothy
Do you mean workload wise, or employer-permission wise? In either case I HOPE that an employer would be willing to give you vacation (or better yet, educational leave) to go to a national meeting for which you will present a project or workshop relating to your job. That is a dream employee!

[Histonet] RE: Extra money

2013-05-30 Thread Mayer,Toysha N
Hey Sarah, Try the local Community college. They may have a need for someone to help with labs on weekends. Also, try a, god forbid I say this, POD labs. They are growing the area and may need the extra expertise. The Clinical Research companies in the area may also be looking. I'm

RE: [Histonet] Extra Money

2013-05-30 Thread joelle weaver
Many reasons over the years. Usually staffing issues. You have a nice attitude about it, I'm sure your employees appreciate that. Joelle Weaver MAOM, HTL (ASCP) QIHC From: timothy.mor...@ucsfmedctr.org To: joellewea...@hotmail.com; sdys...@mirnarx.com; rjbu...@yahoo.com;

RE: [Histonet] Processing thin slices of mouse cerebellum

2013-05-30 Thread Truscott, Tom
Hi Kathleen, Several years and a couple employers ago we attached small biopsies (for proper orientation) to small squares of cucumber (which had been dehydrated in several changes of alcohol) with a egg-albumin/glycerol mixture. The bx/cucumber unit was embedded together after processing and

RE: [Histonet] Extra Money

2013-05-30 Thread Morken, Timothy
Well, here it helps we are a large institution that does give education leave and we do a lot of cross training and have many per diems available. Smaller labs have a much tougher situation. Been there! Tim From: joelle weaver [mailto:joellewea...@hotmail.com] Sent: Thursday, May 30, 2013 9:40

RE: [Histonet] Extra Money

2013-05-30 Thread Sarah Dysart
Well if that's the case I just spent a lot of my companies money for nothing... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: Morken, Timothy

[Histonet] Re: Processing thin slices of mouse cerebellum

2013-05-30 Thread Bob Richmond
Tom Truscott replies to Kathleen [Robert]: Several years and a couple employers ago we attached small biopsies (for proper orientation) to small squares of cucumber (which had been dehydrated in several changes of alcohol) with a egg-albumin/glycerol mixture. The bx/cucumber unit was embedded

RE: [Histonet] Extra Money

2013-05-30 Thread Mitchell Jean A
To clarify: Winning NSH poster presentations in both Clinical and VIR categories each receive $250.00 - but NSH does not reimburse any expenses for poster presentation. Hope no one got their hopes up on this Jean Mitchell -Original Message- From:

[Histonet] HT vs. HTL

2013-05-30 Thread McKenzie, Emily
Can anyone provide job descriptions for HT vs. HTL. We are looking to separated the two positions. If there are any suggestions please let me know. Thank you, Emily McKenzie Memorial Medical Center This message (including any attachments) contains

Re: [Histonet] antigen retrieval question

2013-05-30 Thread Jan Shivers
Lynn, I use the Decloaker and also stain for BVD, so will get in contact with you later this afternoon. Jan Shivers University of Minnesota Veterinary Diagnostic Lab St. Paul, MN 55108 On Thu, May 30, 2013 at 9:54 AM, Burton, Lynn lynn.bur...@illinois.govwrote: If anyone out there is using a

Re: [Histonet] Extra Money

2013-05-30 Thread Lee Peggy Wenk
The poster reimbursement has been addressed. So I'll talk about workshop presenters. It's not a free trip, but some of it is paid. For presenting 1 workshop, you are reimbursed: - air fare or cost of driving, whichever is least - parking at airport (if flying) or at hotel (if driving) - 2