[Histonet] Histology Lab Manager postion openig

2013-05-31 Thread Shelly Christenson
We are currently looking for a Histology Lab Manager at the Veterinary Diagnostic Lab, Kansas State University in Manhattan Kansas. If interested in the position I have listed the advertisement information below or if you want more detailed information on the position you can contact Dr. Jamie

[Histonet] CD45 Ab

2013-05-31 Thread Hobbs, Carl
I need an Ab against CD45 ( LCA) that works in mouse FFPWS. I would be most grateful for a solid recommendation ( you know it works, rather than what is said on spec sheet) Thank you Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College Londo

[Histonet] Re: Inexpensive microwave for staining

2013-05-31 Thread Sherwood, Margaret
To all: I realize that this question has come up before, but what microwave do most labs recommend for special staining? We have never had the need for one, but now do. We would like an inexpensive one. Thanks! Peggy Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photo

RE: [Histonet] RE: Formalin

2013-05-31 Thread Mark Turner
I don't believe there are any regulations on the national or federal level, however you might want to check any local regulations pertaining to storage amounts. I have found these to vary widely, many times being much more strict than national regulations. A 2 gallon maximum is not realistic i

[Histonet] The use of PA's

2013-05-31 Thread Sullivan, Beatrice
To anyone out there utilizing PA's in their histology lab. I am in the early phase of establishing a realistic benchmark for case load per PA. We are a multidivisional facility. Currently there are 3 fulltime PA's. We are not open on weekends or holidays. They do not do autopsies. They do most

Re: [Histonet] RE: Formalin

2013-05-31 Thread darkdaym
Elizabethand Allison, The US adopted the Globally Harmonized System(GHS) some time ago. Compliance is not yet required, but you might want to check out 29CFR1910.1200. It will probably affect all labs to some extent. Just enter that term in your browser. The OSHA GHS storage requirement for

[Histonet] RE: Histonet Digest, Vol 114, Issue 30

2013-05-31 Thread Stafford, Rosemary
Please remove me from the mailing list. Thank you -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Friday, May 31, 2013 1:02 PM To: histonet@lists.utsouthwes

[Histonet] RE: Formalin

2013-05-31 Thread Elizabeth Chlipala
Allison I have not heard of that either, that would mean that you could not even store one 5 gallon cube of formalin. I would politely ask them for a copy of the regulation that states that there can only be 2 gallons of formalin stored in your gross room. They may be classifying the formali

Re: [Histonet] Formalin

2013-05-31 Thread Rene J Buesa
Formalin is THEORETICALLY (and very UNLIKELY) "flammable, but putting it into a flammables cabinet is going too far. Try to find out those conflicting standards (JCAHO vs. CAP) and follow the strictest. Also please remember that many inspectors just "love to show off!" Some think that have to "c

[Histonet] Formalin

2013-05-31 Thread Scott, Allison D
Hello to all in histoland. Happy Friday. Last week we were inspected by a new group of surveyors that are like JCAHO called DNV. They said that we had to much formalin stored in our gross room. We had 15 gallons. They told us that we could only have 2 gallons there at a time. I have never

[Histonet] RE: bone marrow aspirations

2013-05-31 Thread Hannen, Valerie
Diana, We let the aspirates clot in a petri dish, cover them in formalin to fix the clot and then make a block from the fixed clot. Process overnight in the processor and embed the clot in the A.M. This process helps eliminate the aspirate from spreading out in the base mold. I hope this hel

[Histonet] bone marrow aspirations

2013-05-31 Thread Diana McCaig
Can anyone share with me their process for processing bone marrow aspirations and embedding them in paraffin blocks. Currently ours are being spread out throughout the entire base mold and makes it cumbersome to screen. Has anyone used histogel or making a button and embed that instead of just

Re: [Histonet] Steiner stain

2013-05-31 Thread Rene J Buesa
Usually any (and almost all) precipitates when using a silver stain is produced by the silver solution reacting over some remnant of a previous solution not completely removed. Check your solutions, if needed prepare them anew and try again. René J. From: "Herring, Colleen" To: Histonet@lists.u

[Histonet] Autotechnicon processor

2013-05-31 Thread margaret chadwell
Does anyone have an Autotechnicon Tissue Processor for sale?Thanks much!Margie Chadwell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Steiner stain

2013-05-31 Thread Herring, Colleen
I am looking for some suggestions on a problem with our steiner stain. We have a percipitate on the whole slide not just on the tissue and no matter what we have done I can't seem to get rid of it. HELP!

[Histonet] Movats problem solved

2013-05-31 Thread Molinari, Betsy
Good Friday to all. Histology 101. The Alcian Blue dye itself had lost its efficacy. I borrowed a solution from another lab and voila, perfect staining. Thank you to everyone for their suggestions. I will certainly keep them on file in the event this ever happens again. Have a good weekend. Bets

RE: [Histonet] Processing thin slices of mouse cerebellum

2013-05-31 Thread Hobbs, Carl
http://www.cellpath.us.com/magento/cellpath-on-line-shop/specimen-processing-and-embedding/small-biospy-processing/cellsafe/cellsafe-biopsy-capsule-blue.html I find them very good for brain slices. There is a deeper and a shallower space, depending on which way they are folded. Carl Hobbs FIBMS