Since we have some power issues here and 4 Excelsiors (no problems with them on
this issue) we have UPS units on all of our computerized equipment. We did
this due to issues in other areas of the lab and decided it was safer than
taking a chance and being down. We bought Powervar and I think
We have recently started using a Sakura Tissue-tek Prisma that we inherited
from our sister lab. While running some validation testing I have noticed
that the staining is very inconsistent. The tissue will stain from light to
dark within the same rack and sometimes within the same cut (slide). I
I'm cleaning house :) Anyone interested in buying these old books?
1. A color Atlas of Histology by Dennis Strete, ISBN 0-06-501084-1
2. Human Histology, 2nd edition, by Alan Stevens and James Lowe, ISBN
0-7234-2485-3
3. Immunolgy, 4th edition, by Roitt, Brostoff, Male ISBN 0-7234-2178-1
4.
Something to remember is that humidity is the enemy too. If you are storing
your slides in a refrigerator make sure that the humidity is well controlled.
We found out the hard way.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Hi all,
I am in a research lab and I am cutting rat arterial grafts made with ePTFE.
I'm cutting at 30 degrees at 5 microns. The lower edge of the graft seems to
be lifting up from the adventitia and folding over. I have tried playing with
the temperature, but none of it seems to make a
Hi Joel,
Lipids are fixed only by OsO4, so you need to use it as a post-fixative.
As a fixative in our lab we use Karnovsky fixative, then we use osmium
tetroxide as a post-fixative/fixative for the lipids for 15 min. Then we wash
in Na-cacodylate buffer for 5 min, and then dehydrate, starting
This message is for Casey Arnold, we never had any problems with our
stainer/coverslipper, I will send you our procedure to try out. Do you dry
slides on the stainer or elsewhere?
Andrea De Jager, H.T. ASCP
Histology Manager, Regional Reference Lab
Kaiser Permanente - Colorado
Phone:
We currently have an opening for a part-time contingent Pathologists' Assistant
at our Royal Oak, Michigan campus. Please go to
www.beaumont.eduhttp://www.beaumont.edu and click on the careers tab for
more information.
Sharon Scalise, HTL(ASCP)
Histology Supervisor-Anatomic Pathology
