[Histonet] PCR on paraffin sections

Hi. We would like to start doing PCR on paraffin fixed tissues from pigs.. It has some appeal to us because we can see the lesions in the tissue and then we can go after the causative agent post-hoc. It may be useful in cases where we have clear lesions on histopath but not the right fresh

[Histonet] FFPE PCR

ProtK digestion at 55 degrees C for half an hour will do fine There is also a new FISH like technique to detect single RNA molecules in situ, can't remember the name atm. But it works with so called Z-probes and it's very specific and sensitive. Especially when you want to look at virus in

[Histonet] anyone running PAX5 on smears

Hello, histonetters, this feed has proven helpful time after time. Does anyone run PAX5 (currently we are using DAKO antibody on Ventana stainer) on smears? We have it worked up in ffpe, but having some trouble getting staining with the smears. We have been acetone fixing the smears and the