RE: [Histonet] HPV ISH Probes

I believe so, yes. This is the info I was given. Buy Biotin Antibody Rabbit Polyclonal: A150-109A from Bethyl Laboratories, Inc. 25043 West FM 1097, Montgomery, TX 77356, 1-800-338-9579 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

Re: [Histonet] HPV ISH Probes

We have gotten good results with the following probes. http://www.panomics.com/products/rna-in-situ-analysis/viewrna-ish-tissue-assay/overview At first they were not working well but the rep come out for the day and went over them with us and not the results are beautiful and consistent.

RE: [Histonet] HPV ISH Probes

What detection are you using? We only have Ventana instrumentation at our lab. Thank you Cecilia M. Obregon, HTL (ASCP) Memorial Regional Hospital 3501 Johnson Street Hollywood, FL 33021 954-265-5317 From: margaret chadwell [m_chadw...@hotmail.com]

[Histonet] making 10 % EDTA

Hi Histonetters, I have purchased a liquid EDTA solution that is .25M. I need to make 10% EDTA. Do I just dilute it 1/10 with DI water? Thanks Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD

RE: [Histonet] making 10 % EDTA

The MW of EDTA is 294.2. A 1M solution would therefore contain 294.2 g/L, so a .25M solution would contain 73.55 g/L. A 10% solution would contain 100g/L, so your solution is already less concentrated than 10%. ___ Histonet mailing list

[Histonet] Bone marrow blocks

I sent out a request for information a while back and am going to ask again from a different approach. I am finding problems with embedding blocks of bone marrow particles with or without blood clot. Does anyone have a special technique they would like to share to concentrate the particles

[Histonet] Cytology Staining

I have a question for those of you that also may perform cytology specimens at your facility. How do you prevent carryover from one case to another? For example, we had obvious carryover from a pleural fluid onto an FNA of a thyroid case. This has never happened here (to our knowledge). Has

RE: [Histonet] making 10 % EDTA

The molecular weight of the EDTA will vary depending on chemical makeup - regardless, the solution you have is probably less than 10% already. EDTA tri-sodium salt with MW 358 at 0.25 M is 89.5 g per liter or 8.95 g per 100 ml or 8.95%. -Original Message- From:

[Histonet] RE: Bone marrow blocks

Well you could try the warm agar approach: Agar Cell Block Method Reference: Olson NJ, Gogel HK, Williams WL, et al (1986) Processing of aspiration cytology samples: an alternative method. Acta Cytol 30:409-412. Solutions: 1. 3% Agar 2. Fixative Procedure: 1. Fix material in

[Histonet] Histology lab supervisor opening, Connecticut

HISTOLOGY SUPERVISOR Dermatopathology Laboratory of New England seeks Histology Supervisor to join our excellent team. The supervisor oversees all laboratory functions, with the primary role being participation with and supervision of histology technicians in all aspects of processing skin

Re: [Histonet] making 10 % EDTA

Don't forget EDTA doesn't fully go into solution until the pH approaches 8.0. You will need to prepare a sodium hydroxide solution for pH adjustment. On Wed, Jun 26, 2013 at 3:46 PM, Goins, Tresa tgo...@mt.gov wrote: The molecular weight of the EDTA will vary depending on chemical makeup -