[Histonet] CAP REG

Is there a CAP or some Regulation with a percentage for correlating the frozen section diagnosis with the final diagnosis, my pathologist seems to think there is we are joint commision and can't find anything about this, can anyone help?? Thanks Histology/Cytology Supervisor S. Kathy Baldwin,

[Histonet] Cutting Frozen Brain

Hello Everyone, Does anyone have any suggestions for cutting corneal rat brains (20 microns), fixed in PFA, treated with sucrose and embedded into OCT. They cut well but when I pick them up on the slide they always have wrinkles, I have tried many different things with no luck. Different modes

[Histonet] DAB enhancer

I have been asked by a new pathologist to look into a DAB enhancer for use on our Bond III instruments. Are many of you using this on the Bond, or any other staining platform and what are the pros and cons of its use? Thanks!   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab

[Histonet] DAB enhancer

Sorry but I cannot get my messages to go through to the Histonet so I am piggybacking onto a previous message: I have been asked by a new pathologist to look into a DAB enhancer for use on our Bond III instruments. Are many of you using this on the Bond, or any other staining platform and

[Histonet] iNOS

Hello to Everyone, Does anyone have an antibody for iNOS that will work in Human? I appreciate any help you wonderful people could give me. Also, could you share your protocol with me? Thanks, Pat Pat Bell HT(ASCP) Medical Oncology University of Colorado, Denver 12801 E 17th Ave, MS 8117

Re: [Histonet] DAB enhancer

I don't know why anyone would want to use a DAB enhancer for slides prepared on Leica-Microsystems' Bond Max platforms. The resulting immunoreactivity is exceptionally Robust in my experience when using their Bond Polymer Refine Detection kit. Could this request be for a protein target

[Histonet] Ciliated Protozoa

I am way out of my league here and need help from the histonet world. I have been asked to do wet and dry silver nitrate-protargol staining for a research project on ciliated protozoa. I need to speak with someone out there who has done this before. You may contact me directly. Thanks

[Histonet] re wrinkles in section

Hi Margaret, One possible solution to the wrinkling problem you have is to float your twenty microns onto the slide. I do this regularly with mouse or rat brain by cutting the section and putting it into culture wells with buffer solution. Then once I have completed my sectioning I lift the