RE: [Histonet] Losing tissue on IHC slides

2013-07-29 Thread Sue Hunter
Good Morning Another thing to try is different slides. We have had issues with bad batches/lots of slides - even the Fisher Superfrost plus. Especially if your purchasing department buys in quantity and then stores the slides in hot warehouses. This seems to negate the charge on the slides -

[Histonet] HT jobs in Atlanta?

2013-07-29 Thread Rooster
Hello histoneters! Anyone know of any Histology technician job opportunities in the greater Atlanta GA area? Chris ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] HT jobs in Atlanta

2013-07-29 Thread Rooster
Hello Histoneters, Anyone know of any job openings for a registered and experienced histology technician in the greater Atlanta GA area? Thanks Everyone, Chris ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Used Tissue Processor

2013-07-29 Thread Kelli Goodkowsky
Good Morning, I am looking to purchase a used tissue processor in good working condition for our student laboratory. Please contact me if you have something. I would like to have a tissue processor for the fall semester if at all possible. Thank you. Kelli Goodkowsky Director Clinical

[Histonet] Looking for input on tissue processors

2013-07-29 Thread Vickroy, Jim
We are evaluating tissue processors to replace one of our 1980's VIP. So far we have looked at the Thermofisher STP420, Leica ASP6025, VIP6, and we may look at theThermofisher Excelsior ES. I would be interested in the opinions of other customers which processor they would go with and why.

[Histonet] RE: Looking for input on tissue processors

2013-07-29 Thread Marcum, Pamela A
Hi Jim, We have four Excelsiors here at UAMS in the Histology Lab. We bought two to replace very old VIPs over 2 years ago and were very happy with the processing and the safety. We have less exposure to fumes as well as ease of use. As supervisor I am concerned about safety. If I find

RE: [Histonet] HT jobs in Atlanta?

2013-07-29 Thread Terri Brown
Northside Hospital- Histology Supervisor Histology Tech II Histology Tech I Terri H. Brown,, HT (ASCP) Pathology Laboratory Manager Northside Hospital Atlanta terri.br...@northside.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] Recycled Xylene

2013-07-29 Thread Laurie Colbert
Does anyone out there have any problems with using recycled xylene to clean the tissue processor? I was just told that we should not use recycled xylene to clean the tissue processor. How about using recycled xylene for either processing or staining? Laurie Colbert, HT (ASCP) Histology

Re: [Histonet] Recycled Xylene

2013-07-29 Thread Rene J Buesa
I used recycled xylene for any and all tasks where pure xylene was needed. René J. From: Laurie Colbert lcolb...@pathmdlabs.com To: Histonet Post (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu Sent: Monday, July 29, 2013 11:46 AM Subject:

[Histonet] RE: Recycled Xylene

2013-07-29 Thread Nails, Felton
We have been using recycled xylene for years and have found that it only present a problem on the clean cycle of the processor. Everywhere we have not experienced a problem, so we use new xylene on the cleaning cycle. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] Looking for cryomolds

2013-07-29 Thread Carol Torrence
I found an old box of Tissue Tek 4728 cryomolds in our Mohs lab. They are round with a small tab on the side. We like them so much better than the disposable embedding molds sold for paraffin embedding. I have seen cryomolds that look like the same ones for paraffin but are they any thinner

Re: [Histonet] Looking for cryomolds

2013-07-29 Thread margaret chadwell
Hi Carol PSL (Pacific Southwest Lab Supplies) sells the round Cryomolds. Margie Chadwell Sent from Windows Mail From: Carol Torrence Sent: ‎Monday‎, ‎July‎ ‎29‎, ‎2013 ‎9‎:‎18‎ ‎AM To: histonet@lists.utsouthwestern.edu I found an old box of Tissue Tek 4728 cryomolds in our Mohs lab.

RE: [Histonet] Looking for cryomolds

2013-07-29 Thread Diana McCaig
I did not know such a product existed until now. How do these molds work? Diana McCaig Histology Lab Chatham Kent Health Alliance 80 Grand Avenue West Chatham. Ontario N7L 1B7 519-352-6401 (6604) This email communication and any files transmitted with it may contain confidential and or

[Histonet] AM HT

2013-07-29 Thread Bruce Gapinski
Early shift available. We are looking for someone to embed starting at 2:30 AM in gorgeous Marin County California. HT preferred, but will train if necessary. To apply contact Bruce Gapinski bgapin...@pathgroup.com (415) 209-6076 Fax resumes to (415) 898-0870 Bruce Gapinsk HT (ASCP) Chief

[Histonet] used xylene/VIP 6

2013-07-29 Thread Webb, Dorothy L
We have the VIP 6 and really like it..user friendly and faster TAT. We have been having rotary valve concerns within our Leica Peloris tissue processor and the technician as well as Leica technical services both feel it is because we have been using recycled xylene in the cleaning cycle. I was

[Histonet] RE: cryomolds

2013-07-29 Thread Davis, Cassie
The cryomold have been moved on the website: check under specification accessories of the cryostats. I hate when they move things on websites but I guess they are employing the grocery store theory (move thing so customers will see other stuff to buy as they look for what they really need)

Re: [Histonet] used xylene/VIP 6

2013-07-29 Thread Rene J Buesa
Let me be as frank and cynical as possible: properly recycled xylene will produce xylene pure and unadulterated. Those opinions from technical services that also provide non-recycled xylene have a vested interest in making you believe that their product is the ONLY one to use. Never believe

RE: [Histonet] Looking for cryomolds

2013-07-29 Thread joelle weaver
I know the round ones you are talking about. I like them too. The other square shaped are also good. I have some order # 4566, and they are about the dimensions of a metal biopsy base mold with a extended edge. Joelle Weaver MAOM, HTL (ASCP) QIHC From: ctorre...@kmcpa.com To:

[Histonet] Histochoice MB vs other fixatives

2013-07-29 Thread Yun Yung
Dear Histoland, Does anyone have experience using Histochoice for IHC, in particular for mouse brain tissue? Cryoprotected vs paraffinized material? I am currently looking at both structural proteins (e.g., tubulin) as well as transcription factors (e.g., b-catenin and Pax6). I normally use

[Histonet] impact of KOH on melanoma markers

2013-07-29 Thread Gary Gracie
Dear All I recently received a request for HMB45, MART-1, MiTF, S100 and Tyrosinase on sections of toe nail. Our laboratory will usually soften the toe nail in Potassium hydroxide (KOH) either before processing or sometimes after trimming the paraffin block and floating the block in a jar of