Hello
I was just wondering if anyone had a link to any papers discussing the
loss of antigenicity with decomposition. I work with forensic tissue
which is often quite autolysed.
Thanks in advance
Finlay
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Hi CJ,
we had this problem the year before last, it turned out to be the slides.
Apparently, the manufactor had a couple of bad runs. We switched to Avantik
Biogroup Ultra Bond and haven't had a problem since. The bonus was they were
more reasonably priced compare to our contract vendor.
Can someone tell me the CAP approved chemical that can be used to clean the
cryostat?
thanks, Ann
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Ann,
Cryostat decontamination references:
College of American Pathologists, Commentary on certification questionaire:
1) Decontaminate regularly with 70% alcohol.
2) Defrost, remove trimmings and decontaminate with a tuberculocidal
disinfectant, preferably weekly for instruments
For those Histo Guru's who have the VIP tissue processors with the mix
setting of Continuous, slow and fast, what is your perception of the benefit of
using one over the another? What tissues would you use a particular setting?
Why would you NOT use a particular setting?
I know the details of
We use the SLOW exchange setting on all stations. The FAST occurs twice as
often and I didn't want the tissue specimens to be high and dry that many
times during processing. I want an Excelsior . . . . .
Tresa
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Our new lot of Hematoxylin is too dark and especially overstains the mucins in
colon bxs and even membranes/umbilical cord.
Reducing the time from 3 minutes to 1 min helps some, but it the inappropriate
dark staining of the mucins is a real problem. We are also getting a film of
hematoxylin
There was a recent post for someone inquiring about cryomolds. I have not used
these and have researched them and found out the Sakura cryostat has a cryobar
with recessed ports to accommodate these molds. Has anyone used these on a
Microm microtome. Is there a problem removing the molds and
