Probably the same non-effect.Although both procedures are methodologically
different both deal with epitope reactions. The difference is how you visualize
your results.
René J.
From: "Rathborne, Toni"
To: 'Rene J Buesa' ; Teri Johnson ;
"'gu.l...@gmx.at'"
Cc
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Teri:
The automatic coverslipper will wok on oven dried stained sections. I used them
on a Sakura film coverslipper and my lab was in Miami Beach, and you do not
more humid than that!
Xylene is the one weakening the immunoreactivity the most but I have tested the
IPA and the weakening does not
Rene,
You stated that immunoreactivity is not weakened by the use of IPA. Do you know
how the use of IPA might affect FISH results?
Toni
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: T
Teri:
The automatic coverslipper will wok on oven dried stained sections. I used them
on a Sakura film coverslipper and my lab was in Miami Beach, and you do not
more humid than that!
Xylene is the one weakening the immunoreactivity the most but I have tested the
IPA and the weakening does not e
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Great full time opportunity for a Histotechnologist in Morgantown, WV. West
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Thank you Rene. You raise many good points.
I have entertained the idea of eliminating xylene and believe it can be done
successfully. It’s on my list of possible safety-related upgrades to the lab. I
would very much like to get an automatic coverslipper in the lab, but don’t
think that could w
Teri:
It is a wise decision to substitute EthOL with Isopropyl alcohol (IPA) and to
your questions:
1- although it is not advisable to keep tissues for long periods of time in
EthOL sometimes this is done. EthOL will sometimes make the tissue brittle
mostly because its chemical properties but IP
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Hi Teri,
I'm just curious. Is this IPA processing with temperatures about 70°C or
more? Or is it just exchanging ethanol by isopropanol in your usual
processor and protocol?
I ask, because vendors promise no change or even better immunohisto with the
xylenfree/high-temp. method. And I doubt, that
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