[Histonet] RE: goat anti Rabbit Alexa 594 woes.

2013-08-30 Thread gayle callis
You wrote: I would like to get your keen ideas about two issues I am facing now in my immunohistochem. procedure: 1- I am using goat anti rabbit Alexa 594 (red) as secondary antibody for detecting C-Fos in rat nervous system. When I am looking at the slides, it seems always I have some res

[Histonet] AZ HT Position

2013-08-30 Thread Hale, Meredith
Great opportunity for a Histotechnician in a brand new laboratory! Chandler Endoscopy is looking for a certified HT or HTL for their newly constructed laboratory. Candidate must meet the following criteria: * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/

[Histonet] Re: Mouse brain cryosectioning help

2013-08-30 Thread Teri Johnson
Bravo to Donna Emge for her detailed response on this issue. I am really looking forward to seeing the YouTube videos you make. There seems to be a critical lack of these available for histology technique! Best wishes, Teri Johnson Manager, Histology GNF - San Diego, CA 858-332-4752 __

[Histonet] Re: Mouse brain cryosectioning help

2013-08-30 Thread Donna J Emge
Hi Mohammad, For the cracking during cryo sectioning of the mouse brain, that would likely be a temperature issue. Make sure to give the samples just out of a -20 or -80 freezer 15 minutes or longer to adjust to the cryostat temperature before sectioning. Fresh frozen brain typically requires a

[Histonet] VIP and residue problem with Alexa

2013-08-30 Thread Leila Etemadi
Hi every body, I would like to get your keen ideas about two issues I am facing now in my immunohistochem. procedure: 1- I am using goat anti rabbit Alexa 594 (red) as secondary antibody for detecting C-Fos in rat nervous system. When I am looking at the slides, it seems always I have some res