[Histonet] AedestaT-cell/tissue preservation (ACTP) media for muscle bx's

2013-10-04 Thread Sharon Allen
I had read the article on: Nonfrozen Transport Medium Preserves and Restores Skeletal Muscle Enzymatic Activity and Morphology from the The Journal of Histotechnology / Vol. 32, No. 2 / June 2009 posted lately on the Histonet. I was wondering if anyone had used or heard of any further testing

[Histonet] Processing thin brain slices in. Histogel

2013-10-04 Thread kgrobert
We are trying to process thin slices of mouse cerebellum embedded in Histogel into paraffin, and we have encountered two problems: 1) the Histogel button with the slices inside it has been curling up in the processor ( before anyone asks, we're using our own cassettes, not the histoscreen ones

Re: [Histonet] Bodian

2013-10-04 Thread Rene J Buesa
The fundamental problem with the Bodian stain is the silver protargol. Once you open your expensive bottle (only very few manufacturers of the powder) it starts to oxidize end in only a few days it is no longer able to react properly. That is why I developed a procedure where you prepare your

[Histonet] RELIA Solutions Special Career Opportunity Alert Exclusive for Laboratory Directors and Managers !!! 10-03-2013

2013-10-04 Thread Pam Barker
Hello Histonetters!! TGIF!!! RELIA has been engaged by an acute care hospital in beautiful Southern CA (near San Diego) to assist in their search for a director of laboratory services. Previous experience in the role of laboratory management in an acute care setting is essential. Our client

[Histonet] Endogen

2013-10-04 Thread Houston, Ronald
Does anyone know if Endogen has gone out of business or have they been gobbled up by another larger conglomerate? Trying to get one of their antibodies and can no longer find their web-site Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's

[Histonet] Microtome steel blade holder

2013-10-04 Thread Hannen, Valerie
Good Morning all... I am having a problem with my Microtome steel blade holder. One of the screws on the blade block that helps hold the blade tightly clamped has stripped itself out. I am wondering if anyone in out there in Histo-land who no longer is using steel blades, but still has the

[Histonet] Body bags usage.

2013-10-04 Thread Nails, Felton
When you have a patient that expires, do you require that the bodies be brought down in body bags for an autopsy or funeral home pick up? __ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or

Re: [Histonet] Endogen

2013-10-04 Thread koellingr
I recently had the same question. They were gobbled. Try Endogen antibodies at Thermo Scientific conglomerate. Ray Seattle, WA - Original Message - From: Ronald Houston ronald.hous...@nationwidechildrens.org To: histonet@lists.utsouthwestern.edu Sent: Friday, October 4, 2013

[Histonet] RE: Body bags usage.

2013-10-04 Thread Barbara Tibbs
I don't work in a hospital now but when I did, on and off, for the past 30 years or so a body was always brought down to the morgue in a body bag or shroud. If a patient was morbidly obese the body was placed in a bariatric body bag and brought to the morgue. Barbara S. Tibbs Histology

[Histonet] Hep Par 1 validation

2013-10-04 Thread Cynthia Robinson
I am wondering if anyone has hepatoblastomaeither in liver or metastatic tumorin their tissue bank. I am working up Hep Par 1 and have a few cases but would like a few more. We are working up this antibody to use in metastatic case with unknown primary as a way to rule out

RE: [Histonet] Hep Par 1 validation

2013-10-04 Thread Cartun, Richard
FYI - be careful; other cancers besides HCC can show immunoreactivity for Hepatocyte antigen (clone OCH1E5). Arginase (rabbit mAb) is another marker that can be used for HCC and it is more specific than Hepatocyte antigen. Richard Richard W. Cartun, MS, PhD Director, Histology

[Histonet] HistoDeck question

2013-10-04 Thread Mesru T
I have 2nd and 3rd edition of Freida Carson's Histotechnology-A self Instructional text book. When I read the : 2nd edition pg 98, or 3rd edition pps 117-118. I noticed that this is not for OilRed O staining but for forozen sections in general. When I read: Freida Carson's Histotechnology-A self

[Histonet] Finetec Cryofilm?

2013-10-04 Thread Cathie Crukley
There was an earlier email from Elizabeth Cameron looking for information on cryofilm. We have been purchasing cryofilm from a company called Section-Lab in Japan. She would be able to get information on their products from i...@section-lab.jp Cathie Crukley London, Ontario

[Histonet] Re: Microtome steel blade holder

2013-10-04 Thread Brent Rankin
Hi Valerie, I have a couple different knife holders for the 20 series microtomes. Just making sure we are on the same page, you have a standard knife holder and not a disposable blade holder? If you can send me a picture of which one you have I might be able to help you out. Regards, Brent

Re: [Histonet] Bodian

2013-10-04 Thread LuAnn Anderson
Renecould I please get a copy of that procedure ? I would greatly appreciate it. On 10/4/2013 9:35 AM, Rene J Buesa wrote: The fundamental problem with the Bodian stain is the silver protargol. Once you open your expensive bottle (only very few manufacturers of the powder) it

[Histonet] NSH emails

2013-10-04 Thread Sarah Dysart
Does anyone know how to get an email address of someone who presented? If not does anyone have the addresses for Melanie von Brandenstein or Heike Goebel? Thanks Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas

[Histonet] lower glands in colon not staining with IHC

2013-10-04 Thread Cathy Crumpton
On our AE1/AE3 and CK 20 slides we are noticing a deminished staining in the lower glands of the colon sections. My first thought was inadequate fixation, however the BerEP4 stained evenly. This has been happening on different tissues, including small biopsies. Does anyone have any ideas as

[Histonet] Kawamoto's film method

2013-10-04 Thread CSH Newsletter
Hi Everyone, I'm trying to work out sectioning frozen undecalcified mouse bone marrow without using the CryoJane method. The UV light interferes with protease activity. I've been reading about the Kawamoto method and wanted to know people's opinion and how to get hold of the tape. Thanks in

Re: [Histonet] NSH emails

2013-10-04 Thread Lee Peggy Wenk
1. Convention booklet - At the convention, every attendee was given a spiral bound booklet, with thick glossy pages. The last tab is the Speaker Directory with Hospital/lab info, city/state, email addresses. 2. NSH Member Directory - go to NSH webpage (www.nsh.org ), go to Member Directory