[Histonet] Autofluorescence on Retina Tissue

2013-10-15 Thread Allyse Mazzarelli
Hi all! Question for all you that may be more immuno-experienced than I: I've consistently run immunofluorescence on pig retina and I seem to have a severe case of autofluorescence/background. I've played around with primary and secondary antibody ratios, but that doesn't seem to help my case. Th

[Histonet] Re: Cytopathology Quest - alcohols

2013-10-15 Thread Beth Cox
Regarding alcohols in cytology: Amber, you are right about those alcohols being equivalents for *shrinkage rates* (and since size is one of the criteria for cyto, shrinkage rates are important) but that does NOT indicate they perform the same. Reagent alcohol (but not all other denatured al