Hi all!
Question for all you that may be more immuno-experienced than I:
I've consistently run immunofluorescence on pig retina and I seem to have a
severe case of autofluorescence/background. I've played around with primary
and secondary antibody ratios, but that doesn't seem to help my case. Th
Regarding alcohols in cytology: Amber, you are right about those
alcohols being equivalents for *shrinkage rates* (and since size is one
of the criteria for cyto, shrinkage rates are important) but that does
NOT indicate they perform the same.
Reagent alcohol (but not all other denatured al