Hello Histonetters: Our lab has a resident wanting to start a project involving drug effect on tumor size. Since he is wanting to compare tumor size at certain time points after drug treatment (and do IHC staining intensity), he wonders how much affect varying fixation times will have on the tissue. We are not doing the processing or embedding ourselves so we can't control for such variation.
Is this a legitimate concern? If one piece of tissue sits in fix 24 hours before processing/embedding, will the morphology be drastically different than a second piece that sits longer (or shorter) than 24 hours? Is so, would fixing the tissue ourselves for a set time and then putting in some sort of buffer or ethanol before we ship the tissue to the company who embeds it make a difference? The resident is really trying to minimize swelling or excess shrinkage of the tissue. Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet