Has this new paraffin a higher melting point than the former? It may happen
that the block/paraffin is too hard at your cutting temperature to stick.
Does it make ribbons after a while trying?
Gudrun
-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
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I am out of the office until 11/14/2013.
I will be out of the office on Nov 13. If you have a question about an
order or need assistance placing an order, or if the matter is urgent,
please call 877-881-1192 between 8am and 8pm and a VWR Healthcare Service
associate will assist you. For other mat
...Although the cost of batch staining would be high for some of these
reagents. I have asked immunostainer vendors about this and none I have talked
to have done anything along those lines
Tim Morken
Supervisor, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Cent
Felton, we are still doing them manually. Some of them could be done on the
newer batch stainers that have heated reagent stations, but the incubation
periods are pretty long so it may not be practical. At least you would probably
need a dedicated instrument.
Tim Morken
Supervisor, Electron Mi
Are the basic muscle and nerve biopsy stains being performed manually or has
someone automated these stains?
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A cork works well for this purpose too..
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
www.saintjosephsatlanta.org
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Atlanta, GA 30342
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Hospit
Try running a wooden dowel (stick) lightly across your blade, dulling it
slightly. Disposable blades can actually be too sharp sometimes.
Philip Manfre, B.A., HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP45-251
PO Box 4
West Point, PA 19486
215-652-9750
215-993-0383 (f
*I am presently using a paraffin designated as an IM product.*
*We are a facility that cuts only autopsies and have been experiencing alot
of rolling of our sections.*
*We did recently switch to this product, because of cost.*
*Question : Is the paraffin you are using working well on autopsy tiss
I was worried about this when I first started doing DIF's here. All I have is a
-20 -25c freezer. At the direction if our pathologist I was challenged to do it
and watch it closely which is what I have been doing for the past year. When I
am done cutting the frozen sections I place Oct back on t
We use formulae R from Leica for both infiltrating and embedding with excellent
results.
Sent from my iPhone
On Nov 12, 2013, at 10:02 AM, "Matthew D. Roark" wrote:
> What paraffin does everyone like for embedding? We are currently using
> Surgipaths EM-400 but its dirty!Who has a clea
I would try adding 1 or 2 more 100% alcohols before the histoclear.
Sent from my iPhone
On Nov 12, 2013, at 9:32 AM, "Gautier, Nicole M." wrote:
> My lab has been having a problem with "specks" appearing on our slides after
> they come out of Histoclear. Our protocol is to dehydrate in 2 min
I will be out of the office starting 11/12/2013 and will not return until
11/30/2013.
Please contact - healthcareservice.com or customer service at
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Hi,
I use a 0.2% phosphomolybdic acid step when I'm staining cardiac muscle.
Just a quick (2 minutes) before the picrosirius red step.
Without the phosphomolybdic acid sometimes the myocytes stain really
yellow and that makes it more difficult to see the type 1 collagen.
If you don't do the phopho
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