RE: [Histonet] Specimen collection/transportation

The usual procedure where I have worked is that the placenta is delivered fresh to pathology in a large container, or refrigerated until next specimen pick up and formalin is added by the lab staff ( who have PPE, formalin training, spill kit) etc. Joelle Weaver MAOM, HTL (ASCP) QIHC

[Histonet] Vendor suggestions for IgG subclass antibodies

I am looking for a vendor for FITC labeled IgG1, IgG2 IgG3 and IgG4 antibodies for use in our immunofluorescence panels when staining renal biopsies. We get most of our FITC antibodies for our panel from Dako but I didn't see these others. Any suggestions are appreciated. Thanks in

[Histonet] Methyl Carnoy's processing

Hello, I have a researcher who has fixed their specimens in methyl Carnoy's but would like to skip the methyl benzoate step before processing (we do not have methyl benzoate). Does anyone have another method that works well without the methyl benzoate? Thank you Hans B Snyder Histologistics 60

[Histonet] Leica RM2255 block clamp

Hello Hoping someone might be able to help with this microtome issue. I have a problem with the block clamp on one of my Leica RM2255's. It has recently loosened up and it is now possible to move the clamped cassette from side-to-side when the clamp is shut. When I turn the bolt on the

[Histonet] New CPT code for IHC

How will everyone be handling the new CPT code 88343 for IHC going into effect 1/1/14? The CPT code 88342 has a new description stating it is to be used for the first antibody and 88343 is to be used for each additional separately identifiable antibody. The concern is that each antibody at

Re: [Histonet] New CPT code for IHC

88343 refers to subsequent antibodies placed on the same slide such as multiplex staining or the PIN-4. Hope that helps. Will Chappell, HTL(ASCP), QIHC Sent from my iPhone On Nov 19, 2013, at 8:31 AM, Leann M. Murphy lmurp...@aultman.com wrote: How will everyone be handling the new CPT

[Histonet] omnimap/chromomap kit for ventana

Hello Fellow Histonetters, I find myself in a bit of a bind. I have a couple of urgent studies/cases that need to be done by the end of this week, but our kit has run out and is on back-order from Ventana. I need to do Caspase and Major Basic Protein (both rabbit antibodies). Would it be

RE: [Histonet] New CPT code for IHC

I think that everyone is waiting to see if CMS makes a policy Change to accommodate 88342 +88343 code changes. The first indication may be via the 2014 Medicare Physician Fee Schedule final rule due out for release on or before November 27, or through the 2014 version of the NCCI Policy Manual,

[Histonet] microtome

Hello, I have undecalcified biological sample embedded in plastic media (MMA). I am looking for faclities that offers self-service microtome (for plastic embedding sectioining) or short time rental microtome with some training around Quebec, Ontraio, NY. I have been trying to find ones in

[Histonet] problem with double IF of brain section

Hello everyone, I’m trying a double stain for GFP and tyrosine hydroxylase in floating brain sections. Rat, perfused with formalin. I’m having a big problem, in that it’s clear that the top side of the sections are well stained, as are the bottom, but everything in between is not. These

RE: [Histonet] omnimap/chromomap kit for ventana

I have two chromo map kits for the Discovery I can send you. Lynn M Burton Histology Animal Disease Lab Galesburg, Il 309-344-2451 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Catherine Simonson

[Histonet] Thank you all.

I wanted to thank everybody, especially Lynn Burton, for their help during my time of crisis. It is nice to be a part of a group that is so supportive and willing to lend a hand. Again, thank you. Catherine ___ Histonet mailing list

RE: [Histonet] Thank you all.

Searching for the like button!! We do have an awesome group! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of

[Histonet] problem with double IF of brain section

Caroline, Overnight at 4 degrees may not be long enough for equilibration of the antibody binding reaction deep in the section. Try room temp or longer time if cold is important. The early users of IHC often used 4 days at 4 deg C, based on antibody binding kinetics data. I get

[Histonet] -80 freezer question

Dear Netters, What is the rationale for storing frozen patient samples at -80? Is there a range that keeps the sample viable but not as cold? Our -80 (which we just inherited) is struggling and I would like to raise the temperature a smidge to ease the stress on the compressor. Thanks! Paula