[Histonet] rat's hind paw skin

Hello every body, First I would like to thank you all for your great feedbacks and attention which is such a big improvement in my work, Thank you all! Then, I was wonder if any one has experience on this tissue: rat hind paw skin ( which is hairless). Any inputs will be appreciated, whether

[Histonet] Labeling Error Policies

Our laboratories are currently working on policies to address specimen identification/labeling errors. This is to be a laboratory policy and will include pathology. Would anyone be willing to share policies that they have that address this and any punitive action that is mentioned? I know

RE: [Histonet] Rate of microtome injuries in Histotechnology schools.

If anyone wants to cut down on cutting injuries. Teach the new techs to use two forceps instead of your fingers. I'm too old to change but I know a tech who cuts this way and has never had an injury. It works really well on automated microtomes. Anne -Original Message- From:

[Histonet] Embedding cells in Paraffin

Has anyone tried to embed cells grown in tissue culture? I am trying to put some tissue culture cells through same stress as tissue would go through. Fixation, dehydration, and heat. Any ideas? I could re-suspend in OCT and then fix for extended time with NBF but that doesn't quite seem fair.

[Histonet] embedding cells in parrafin #2

Making cell tissue blocks Cells: . Harvest and fix in formalin* for at least 3hrs or overnight (10 fold more formalin) . HAVE A NICE VISIBLE SIZE PELLET (1 confluent T75 minimum or 1 T175) . Remove formalin* and wash 2x's in cold 1x PBS . Cover cells with some 1x PBS and

[Histonet] Purple/Blue haze on ISH

Hi all... sending out for a colleague... After implementing the Vantage system, a lot of their ISH slides have a purplish to bluish haze on the slides. Not all of them, but some and it seems to be consistent. No problems before Vantage. They were using the longer labels from Ventana for

[Histonet] RE: Purple/Blue haze on ISH

Karen, We experienced a similar problem with the ISHs at our laboratory a few months ago with the Kappa, Lambda, Eber and HPV. Our pathologists were complaining of a blue crystal-like precipitate on all ISH slides. Our Ventana Technical Specialist recommended that we try the NO ALCOHOL

RE: [Histonet] Embedding cells in Paraffin

I spun down the cultured cells and then used a product called histogel. It kept them protected through the VIP, paraffin embedded like cell block buttons and was able to cut regular paraffin sections. I believe that I purchased the histogel from American Mastertech. Joelle Weaver MAOM, HTL

[Histonet] RE: Embedding cells in Paraffin

Mike, For EM work we fix, scrape the plate into a microtube, centrifuge at 1800 rpm for 10 min and then embed in Histogel or agar. Processing after that is as usual. They look fine by EM. I'm sure they would look fine by paraffin processing as well. Tim Morken Supervisor, Electron Microscopy

[Histonet] Donations Needed

Dear Histo Folks, I am in the process of setting up a brand new histology/IHC laboratory on the campus of the Uganda Cancer Institute in Kampala, Uganda in conjunction with the Fred Hutchinson Cancer Research Center. As you can imagine, we need anything and everything as supplies and small

[Histonet] anti-Human CD20

Thanks for all the great replies!! While I have you all worried about my cell pellets ;-) Does anyone have a good anti-HuCD20 that works well for FFPE tissues (cells). Thanks! Mike ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] RE: See you Soon

Congratulations Sarah Sincerely, Toysha N. Mayer, MBA, HT(ASCP) tnma...@mdanderson.org Instructor/Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center 713-563.3481 Message: 1 Date: Wed, 27 Nov 2013 19:13:05 + From: Sarah Dysart

[Histonet] Grossing tables

I'm currently designing a new GI lab. Any advice on grossing stations? The only name I know is Mopec. Is that the best one to go with or are there better ones out there? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] RE: Grossing tables

I think that's the best - you can charge for rides - up and down!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the

RE: [Histonet] RE: Grossing tables

http://www.thermoscientific.com/en/products/pathology-work-stations.html Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax)

[Histonet] Re: [IHCRG] macrophage eq markers?

In which animals works also this antibody ? Thanks On Mon, Dec 2, 2013 at 11:20 PM, Jan Shivers shive...@umn.edu wrote: Dako's MAC 387 (cat. #: M0747) On Mon, Dec 2, 2013 at 2:09 PM, Jackie Ferracone ferra...@vet.upenn.eduwrote: Hi All, Looking for good macrophage markers for equine

[Histonet] Chris van der Loos

Sad news. I received an email over the weekend from a histotech, that Chris van der Loos died on Nov. 26, 2013. I was able to talk with the NSH office today, and got it confirmed. There is information about Chris on the NSH webpage as of later this morning.

[Histonet] Re: [IHCRG] macrophage eq markers?

I've had it to work in cat, cow, dog, fox, horse, otter, pig, and raccoon in my lab. According to vendor - it's supposed to also work in guinea pig, monkey, rabbit, and rat, but I have not tried it in those animals Needs either enzyme digestion or heat antigen retrieval. Best regards, Jan

RE: [Histonet] Chris van der Loos

Very sad news indeed. He will be missed dearly! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] Chris van der Loos

This is very sad. Chris was knowledgeable, with a wonderful sense of humour and always had time for his colleagues. He will be missed Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist, the Children's Hospital at Westmead Adjunct

[Histonet] Chris van der Loos

I had the privilege and good fortune of getting to know and swap ideas with Chris after spending a few weeks with him in Breda back in 2001. Never short of advice and always willing to help and learn from others. To use an Australian expression he was an absolute Cracker of a guy. Cheers Jim

[Histonet] RE: Chris van der Loos

I agree Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the

Re: [Histonet] rat's hind paw skin

Hi Leila As no one seems to have responded to your email I will add a few items. I used to do some work on wound healing on mouse footpads so circumstances are the same. If you are looking for melanin routine fixation and processing should be finem, melanin will remain. Only thing is that the

[Histonet] (no subject)

Hi, There are a few ways of doing this. Perhaps the easiest is scraping the cells off while it is still in the media. Transfer it to a centrifuge tube and spin it down. Pour off the supernatant add formalism vortex it and centrifuge it again and pour off the supernatant again and add