[Histonet] RE: Questions about IHC in Frozen Sections

Patrick Here is what we do for frozen IHC, this is based upon methods that I received from Gayle Callis. Cut frozen sections and let air dry - at least 20-30 minutes post the last section cut. If we are going to stain that same day or the following day we leave the slides at room temp (we are

[Histonet] RE: Yahoo link

We are finally recognized and it is for this. So sad :( -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Tuesday, December 03, 2013 6:31 AM To: histonet@lists.utsouthwestern.edu (histonet@li

[Histonet] CAP Survey Question

Hi, Regarding the question of weather it would save time to not have the techs QC check the IHC slides. I feel that it would be an extremely bad practice to omit the techs from the QC process. This goes for not only IHC but special stains and even H&E stains. It is imperative that the techs

Re: [Histonet] The Twelve Days of Histo

Thaks waiting for the  ice storm and sleeping in the lab tonight so we appreciated a good laugh.  Did you notice you have to sing it in your head? Pam Marcum - Original Message - From: "Kenneth A Troutman" To: Histonet@lists.utsouthwestern.edu Sent: Thursday, December 5, 2013

[Histonet] RE: The Twelve Days of Histo

That is great! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Garcia, Lori, M.Sc. Sent: Thursday, December 05, 2013 2:09 PM To: Troutman, Kenneth A; Histonet@lists.utsouthwestern.edu Subject: [Histonet] R

[Histonet] Questions about IHC in Frozen Sections

Hi Everyone. I am trying to troubleshoot my IHC on frozen sections. My sections are human tonsil at 7 uM. On charged Superfrost slides. They are stored at -80 after drying for 1 hour. When I use them for IHC, I take them out of the -80 and let them air dry for 1 hour before placing them in co

[Histonet] RE: The Twelve Days of Histo

Love it :) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Troutman, Kenneth A Sent: Thursday, December 05, 2013 2:03 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] The Twelve Days of Histo

[Histonet] The Twelve Days of Histo

Hello all, I hope the holidays are treating everyone well. (Especially in light of us having the most hazardous job in the country...) Here's some fun for the season: The Twelve Days of Histo TWELVE Medical Directors ELEVEN logs-a-printing TEN residents reading NINE frozen sections EIGHT admi

[Histonet] BAP1

Anyone doing IHC "BAP1" for cutaneous melanocytic tumours, mesothelioma, Spitz tunours. I think Santa Cruz has the antibody. Any tips, tricks or info on the protocol. Thanks in advance. Michael, great to talk with you ! Maryann Deathridge, BS, HT (ASCP) Lab Manager 615-298-4100, Fax

[Histonet] RE: CDH1

Found it at Stanford - so nevermind!! Thanks, j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph'

RE: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde

i would think u are correct in advising formic acid decal and then processing into paraffin for the best protection of the trap enzyme, immunoreactivity, etc. A couple of weeks in formalin should be fine. Paraformaldehyde show be the same as formalin. I do know a way to restore the enzyme act

[Histonet] CDH1

Anyone doing this mutation test by PCR - performed on normal tissue for families with highly penetrant hereditary diffuse gastric cancer? Thanks, j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org This e-mail m

Re: [Histonet] histology tracking systems for pharma or contract lab

xybion is in the process of developing a histology module for Phama histo. We at AbbVie will be using this system. Jackie Sent from my iPhone > On Dec 5, 2013, at 13:23, Kim Merriam wrote: > > Hi, > > My company is considering a new tracking system for our histology studies. > This system

Re: [Histonet] Helicobacter helmani -- help?

All - thank you for the education! We're waiting for a definite response from the manufacturer and will offer an Alcian yellow while we wait... Happy holidays!! Sent from my iPhone > On Dec 4, 2013, at 6:01 PM, Milanka Ilic wrote: > > Hi Cheryl, > > The polyclonal Helicobacter pylori (

[Histonet] histology tracking systems for pharma or contract lab

Hi, My company is considering a new tracking system for our histology studies.   This system needs to track everything from trimming, decal, embedding, cutting, routine and molecular pathology/staining.  It also needs to be able to accommodate complex study designs (multiple animals, groups, nec

[Histonet] Position in Rosemont, IL - DIRECTOR CENTRAL LABORATORY - HISTOLOGY

Hello Fellow Histonetters, I want to let everyone know about this new position for a Histology Director at ACL laboratories in Rosemont, IL, near Chicago. I just joined ACL a couple of weeks ago. It is very dynamic and a great place to work. Donna Emge, HT(ASCP) Histology Supervisor

AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde

Paraformaldehyd is formaldehyd in solid form. Formalin is the aequous solution of formaldehyd. So the main characteristics are the same. Gudrun Lang -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von

[Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde

Dear Histonetters, What is your opinion on storing bone samples long-term (more than a couple of weeks) in 10% formalin? As I was taught, best practice has always been to fix only as long as necessary, depending on the size of the sample, then decalcify and process to wax, and I always stress this

RE: [Histonet] Shandon Grosslab Senior

Try Mercedes Medical Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Ma

RE: [Histonet] Shandon Grosslab Senior

Christi, I just built a lab and ordered 5 of them through Mopec, they were fantastic to work with and gave discounts for each additional unit purchased throughout the 12 month period that I bought them. Michael Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology L

RE: [Histonet] Shandon Grosslab Senior

Mercedes Medical sells their own brand that are compatible with others out there and sell for less than Thermo. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi Stephenson Sent: Thursday, December

[Histonet] Shandon Grosslab Senior

Hello, Does anyone that uses this grossing station have a great (cheaper than ThermoScientific) place to purchase filters? Thanks, Cristi ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histon

[Histonet] Aviva antibodies

Does anybody know about the quality of Aviva Systems Biology antibodies for IHC ? Thanks for your help. Julie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] M2D2 and SMAD

Hi, SMAD4 is very difficult on the Ventana platform. The only clone that would work on the Ventana to my knowledge(clone EP618Y, immunologic) has recently 'collapsed' (i hope that's the right word?) It means that it is not available anymore. Maybe there is some vendor that has a few vials left

[Histonet] M2D2 and SMAD

Good morning Does anyone have suggestions for antibodies for M2D2 and SMAD? We have not been able to get these antibodies to work for us on the Ventana Ultras. If you do have suggestions, protocols and control tissue suggestions would also be appreciated. Thanks Sue Sue Hunter, Supervisor Adva

[Histonet] RE: CAP survey Question

We do the same process as Linda does here at Beaumont. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.

[Histonet] RE: IHC Buffer (wash)

Try ScyTek - The ready-made price is nearly as cheap as I can make it myself. http://www.scytek.com/default.asp Tresa Tresa Goins Histopathology Section Supervisor Montana Veterinary Diagnostic Lab -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histo

[Histonet] RE: Stainer/coverslipper question

A few months ago I implemented the Lieca stainer with coverslipper, great pricing on the unit! It works well, the coverslipper can be a little touchy at first but once you break it in, it works well...we went to imagining some slides and wanted glass coverslips. On another note, I just impleme